TY - JOUR
T1 - Synthesis of New macromer Based on HEMA-TA-PEG for Preparation of Bio-Based Hydrogels for Regenerative Medicine Applications
AU - Pisheh, Hossein Rayat
AU - Sabzevari, Alireza
AU - Ansari, Mojtaba
AU - Kabiri, Kourosh
AU - Eslami, Hossein
AU - Koohestanian, Mohammad
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025
Y1 - 2025
N2 - In this study, bio-based hydrogels were designed and synthesized using the HEMA-TA-PEG macromer. The chemical structure of the macromer was confirmed through FTIR and 1H-NMR analyses. Hydrogels with varying crosslinker (GPTMS) content were fabricated to investigate the impact of GPTMS on swelling, gel content, and mechanical properties. As the GPTMS content increased, the swelling behavior of the hydrogels decreased, whereas the gel content increased. Mechanical testing revealed a significant increase in tensile strength, with stress rising from 517 kPa to 786 kPa and the tensile modulus reaching 1.03 MPa as the GPTMS content increased from 0.25 g to 1 g. The prepared hydrogels exhibited excellent cytocompatibility, with significantly higher cell viability than the control over time (24, 48, and 72 h). Furthermore, cell adhesion in the hydrogels reached 93% at 48 h, a significant increase compared to the 85% observed in the control. These findings highlight the potential of bio-based hydrogels for applications in regenerative medicine.
AB - In this study, bio-based hydrogels were designed and synthesized using the HEMA-TA-PEG macromer. The chemical structure of the macromer was confirmed through FTIR and 1H-NMR analyses. Hydrogels with varying crosslinker (GPTMS) content were fabricated to investigate the impact of GPTMS on swelling, gel content, and mechanical properties. As the GPTMS content increased, the swelling behavior of the hydrogels decreased, whereas the gel content increased. Mechanical testing revealed a significant increase in tensile strength, with stress rising from 517 kPa to 786 kPa and the tensile modulus reaching 1.03 MPa as the GPTMS content increased from 0.25 g to 1 g. The prepared hydrogels exhibited excellent cytocompatibility, with significantly higher cell viability than the control over time (24, 48, and 72 h). Furthermore, cell adhesion in the hydrogels reached 93% at 48 h, a significant increase compared to the 85% observed in the control. These findings highlight the potential of bio-based hydrogels for applications in regenerative medicine.
KW - 2-hydroxyethyl methacrylate
KW - Biohydrogel
KW - Biomaterial
KW - Regenerative medicine
KW - Tartaric acid
U2 - 10.1007/s10924-025-03584-7
DO - 10.1007/s10924-025-03584-7
M3 - Journal article
AN - SCOPUS:105005092132
SN - 1566-2543
VL - 33
SP - 3076
EP - 3091
JO - Journal of Polymers and the Environment
JF - Journal of Polymers and the Environment
ER -