Synthesis of β-1,4-Linked Galactan Side-Chains of Rhamnogalacturonan I

Mathias Christian Franch Andersen, Stjepan Kracun, Maja Rydahl, William Willats, Mads Hartvig Clausen

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Abstract

The synthesis of linear- and (1→6)-branched-β-(1→4)-D-galactans, side chains of the pectic polysaccharide rhamnogalacturonan I is described. The strategy relies on iterative couplings of n-pentenyl disaccharides followed by a late stage glycosylation of a common hexasaccharide core. Reaction with a covalent linker and immobilization on NHS-modified glass surfaces allows for the generation of carbohydrate microarrays. The glycan arrays enables the study of protein-carbohydrate interactions in a high throughput fashion, here demonstrated with binding to mAbs and CBMs.
Original languageEnglish
JournalChemistry: A European Journal
Volume22
Issue number33
Pages (from-to)11543-11548
Number of pages6
ISSN0947-6539
DOIs
Publication statusPublished - 2016

Keywords

  • Carbohydrates
  • Glycosylation
  • Oligosaccharides
  • Plant cell walls
  • Rhamnogalacturonan

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