The synthesis of linear- and (1→6)-branched-β-(1→4)-D-galactans, side chains of the pectic polysaccharide rhamnogalacturonan I is described. The strategy relies on iterative couplings of n-pentenyl disaccharides followed by a late stage glycosylation of a common hexasaccharide core. Reaction with a covalent linker and immobilization on NHS-modified glass surfaces allows for the generation of carbohydrate microarrays. The glycan arrays enables the study of protein-carbohydrate interactions in a high throughput fashion, here demonstrated with binding to mAbs and CBMs.
- Plant cell walls