Synovitis biomarkers

ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis

Cecilie Freja Kjelgaard-Petersen, Anne Sofie Siebuhr, Thorbjorn Christiansen, Christoph Ladel, Morten Karsdal, Anne-Christine Bay-Jensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNF alpha significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p <0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p <0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.
Original languageEnglish
JournalCancer Epidemiology, Biomarkers & Prevention
Volume20
Issue number8
Pages (from-to)547-556
Number of pages10
ISSN1055-9965
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Synovial membrane
  • Personalized medicine
  • Neo-epitope biomarkers

Cite this

Kjelgaard-Petersen, Cecilie Freja ; Siebuhr, Anne Sofie ; Christiansen, Thorbjorn ; Ladel, Christoph ; Karsdal, Morten ; Bay-Jensen, Anne-Christine. / Synovitis biomarkers : ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis. In: Cancer Epidemiology, Biomarkers & Prevention. 2016 ; Vol. 20, No. 8. pp. 547-556.
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title = "Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis",
abstract = "Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNF alpha significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p <0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p <0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.",
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author = "Kjelgaard-Petersen, {Cecilie Freja} and Siebuhr, {Anne Sofie} and Thorbjorn Christiansen and Christoph Ladel and Morten Karsdal and Anne-Christine Bay-Jensen",
year = "2016",
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language = "English",
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Synovitis biomarkers : ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis. / Kjelgaard-Petersen, Cecilie Freja; Siebuhr, Anne Sofie; Christiansen, Thorbjorn; Ladel, Christoph; Karsdal, Morten; Bay-Jensen, Anne-Christine.

In: Cancer Epidemiology, Biomarkers & Prevention, Vol. 20, No. 8, 2016, p. 547-556.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Synovitis biomarkers

T2 - ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis

AU - Kjelgaard-Petersen, Cecilie Freja

AU - Siebuhr, Anne Sofie

AU - Christiansen, Thorbjorn

AU - Ladel, Christoph

AU - Karsdal, Morten

AU - Bay-Jensen, Anne-Christine

PY - 2016

Y1 - 2016

N2 - Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNF alpha significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p <0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p <0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.

AB - Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNF alpha significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p <0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p <0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.

KW - Synovial membrane

KW - Personalized medicine

KW - Neo-epitope biomarkers

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DO - 10.3109/1354750X.2015.1105497

M3 - Journal article

VL - 20

SP - 547

EP - 556

JO - Cancer Epidemiology, Biomarkers & Prevention

JF - Cancer Epidemiology, Biomarkers & Prevention

SN - 1055-9965

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