Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis

Cecilie Freja Kjelgaard-Petersen, Anne Sofie Siebuhr, Thorbjorn Christiansen, Christoph Ladel, Morten Karsdal, Anne-Christine Bay-Jensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNF alpha significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p <0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p <0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.
Original languageEnglish
JournalCancer Epidemiology, Biomarkers & Prevention
Volume20
Issue number8
Pages (from-to)547-556
Number of pages10
ISSN1055-9965
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Synovial membrane
  • Personalized medicine
  • Neo-epitope biomarkers

Fingerprint Dive into the research topics of 'Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis'. Together they form a unique fingerprint.

Cite this