Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes

Jesper Davidsen, O.G. Mouritsen, K. Jørgensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The permeability-enhancing effects of the two surfactants, 1-paltnitoyl-2-lyso-sn-gycero-3-pllosplloclloline (lysoPPC) and palmitic acid (PA), on lipid membranes that at physiological temperatures are in the gel, fluid, and liquid-ordered phases were determined using the concentration-dependent self-quenching properties of the hydrophilic marker, calcein. Adding lysoPPC to lipid membranes in the gel-phase induced a time-dependent calcein release curve that can be described by the sum of two exponentials, whereas RA induces a considerably more complex release curve. However, when lysoPPC and PA were added simultaneously in equimolar concentrations, a dramatic synergistic permeability-enhancing effect was observed. In contrast, when both lysoPPC and PA are added to liposomal membranes that are in the fluid or liquid-ordered phases, no effect on the transmembrane permeation of calcein was observed.
Original languageEnglish
JournalB B A - Biomembranes
Volume1564
Issue number1
Pages (from-to)256-262
ISSN0005-2736
DOIs
Publication statusPublished - 2002

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