Synergistic enzyme assisted release of ferulic acid from brewer´s spent grain

Paula L. Bucci, Pedro Martins, Raul Muñoz, Anne S. Meyer*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)


Brewer’s spent grain (BSG), the most abundant by-product of the beer industry, can be considered a sustainable source of ferulic acid (4-hydroxy-3-methoxycinnamic acid) (FA). FA is esterified to the arabinoxylan in the BSG. FA exhibits a variety of biological activities and is a target for valorization of BSG. We present a novel enzyme-assisted approach that utilizes the synergistic action of specific endo-1,4-β-xylanase and ferulic acid esterase activity to selectively release high yields of FA from BSG. The study compares the efficacy of three different fungal feruloyl esterases (E.C. of carbohydrate esterase family 1 (CE1), HiFae, AoFae1, and AnFae1, acting separately or in combination with two different endo-1,4-β-xylanases (EC, a bacterial GH30 enzyme from Dickeya chrysanthemi, and Shearzyme®, a commercial GH10 enzyme from Aspergillus aculeatus, respectively. On an equimolar dosage basis, the AnFae1-GH30 was the most efficient combination in releasing FA from BSG. This combination showed significant synergy in a dose-dependent manner, enabling up to 85% FA recovery from BSG within 30 min. of treatment. The results demonstrate a novel enzymatic approach for valorization of BSG as FA is considered a high-value natural compound for use in e.g. anti-aging skin care products.
Original languageEnglish
JournalProcess Biochemistry
Pages (from-to)225-233
Publication statusPublished - 2024


  • Brewer's spent grain (BSG)
  • Enzymatic release
  • Feruloyl esterases
  • GH30 endo-1,4-β-xylanase


Dive into the research topics of 'Synergistic enzyme assisted release of ferulic acid from brewer´s spent grain'. Together they form a unique fingerprint.

Cite this