Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial

Gabriella Christina van Zanten; Lukasz Krych; Henna Roytio; Sofia Forssten; Sampo J. Lahtinen; Waleed Abu Al-Soud; Søren Sørensen; Birte Svensson; Lene Jespersen; Mogens Havsteen Jakobsen

doi:10.1111/1574-6941.12397

Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial

Gabriella Christina van Zanten, Lukasz Krych, Henna Roytio, Sofia Forssten, Sampo J. Lahtinen, Waleed Abu Al-Soud, Søren Sørensen, Birte Svensson, Lene Jespersen, Mogens Havsteen Jakobsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n=18) were enrolled in a double-blinded, randomized, and placebo-controlled cross-over study and received synbiotic [Lactobacillus acidophilus NCFM (10(9)CFU) and cellobiose (5g)] or placebo daily for 3weeks. Fecal samples were collected and lactobacilli numbers were quantified by qPCR. Furthermore, 454 tag-encoded amplicon pyrosequencing was used to monitor the effect of synbiotic on the composition of the microbiota. The synbiotic increased levels of Lactobacillus spp. and relative abundances of the genera Bifidobacterium, Collinsella, and Eubacterium while the genus Dialister was decreased (P

Original language	English
Journal	F E M S Microbiology Ecology
Volume	90
Issue number	1
Pages (from-to)	225-236
ISSN	0168-6496
DOIs	https://doi.org/10.1111/1574-6941.12397
Publication status	Published - 2014

Keywords

MICROBIOLOGY
IRRITABLE-BOWEL-SYNDROME
FECAL MICROBIOTA
METABOLIC-ACTIVITY
HEALTHY-VOLUNTEERS
RHAMNOSUS KY-3
IN-VITRO
FERMENTATION
SUPPLEMENTATION
INTERVENTION
CONSUMPTION
synbiotics
microbial community sequencing
branched-chain fatty acids
Lactobacillus
Bifidobacterium
human trial

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/1574-6941.12397

OpenUrl availability

Full text

Fingerprint

Dive into the research topics of 'Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial'. Together they form a unique fingerprint.

Cite this

van Zanten, G. C., Krych, L., Roytio, H., Forssten, S., Lahtinen, S. J., Abu Al-Soud, W., Sørensen, S., Svensson, B., Jespersen, L., & Jakobsen, M. H. (2014). Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial. F E M S Microbiology Ecology, 90(1), 225-236. https://doi.org/10.1111/1574-6941.12397

van Zanten, Gabriella Christina ; Krych, Lukasz ; Roytio, Henna et al. / Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial. In: F E M S Microbiology Ecology. 2014 ; Vol. 90, No. 1. pp. 225-236.

@article{06e17f84467c4559adc3bfe714c0f46e,

title = "Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial",

abstract = "Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n=18) were enrolled in a double-blinded, randomized, and placebo-controlled cross-over study and received synbiotic [Lactobacillus acidophilus NCFM (10(9)CFU) and cellobiose (5g)] or placebo daily for 3weeks. Fecal samples were collected and lactobacilli numbers were quantified by qPCR. Furthermore, 454 tag-encoded amplicon pyrosequencing was used to monitor the effect of synbiotic on the composition of the microbiota. The synbiotic increased levels of Lactobacillus spp. and relative abundances of the genera Bifidobacterium, Collinsella, and Eubacterium while the genus Dialister was decreased (P",

keywords = "MICROBIOLOGY, IRRITABLE-BOWEL-SYNDROME, FECAL MICROBIOTA, METABOLIC-ACTIVITY, HEALTHY-VOLUNTEERS, RHAMNOSUS KY-3, IN-VITRO, FERMENTATION, SUPPLEMENTATION, INTERVENTION, CONSUMPTION, synbiotics, microbial community sequencing, branched-chain fatty acids, Lactobacillus, Bifidobacterium, human trial",

author = "{van Zanten}, {Gabriella Christina} and Lukasz Krych and Henna Roytio and Sofia Forssten and Lahtinen, {Sampo J.} and {Abu Al-Soud}, Waleed and S{\o}ren S{\o}rensen and Birte Svensson and Lene Jespersen and Jakobsen, {Mogens Havsteen}",

year = "2014",

doi = "10.1111/1574-6941.12397",

language = "English",

volume = "90",

pages = "225--236",

journal = "F E M S Microbiology Ecology",

issn = "0168-6496",

publisher = "Oxford University Press",

number = "1",

}

van Zanten, GC, Krych, L, Roytio, H, Forssten, S, Lahtinen, SJ, Abu Al-Soud, W, Sørensen, S, Svensson, B, Jespersen, L & Jakobsen, MH 2014, 'Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial', F E M S Microbiology Ecology, vol. 90, no. 1, pp. 225-236. https://doi.org/10.1111/1574-6941.12397

Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial. / van Zanten, Gabriella Christina; Krych, Lukasz; Roytio, Henna et al.
In: F E M S Microbiology Ecology, Vol. 90, No. 1, 2014, p. 225-236.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial

AU - van Zanten, Gabriella Christina

AU - Krych, Lukasz

AU - Roytio, Henna

AU - Forssten, Sofia

AU - Lahtinen, Sampo J.

AU - Abu Al-Soud, Waleed

AU - Sørensen, Søren

AU - Svensson, Birte

AU - Jespersen, Lene

AU - Jakobsen, Mogens Havsteen

PY - 2014

Y1 - 2014

N2 - Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n=18) were enrolled in a double-blinded, randomized, and placebo-controlled cross-over study and received synbiotic [Lactobacillus acidophilus NCFM (10(9)CFU) and cellobiose (5g)] or placebo daily for 3weeks. Fecal samples were collected and lactobacilli numbers were quantified by qPCR. Furthermore, 454 tag-encoded amplicon pyrosequencing was used to monitor the effect of synbiotic on the composition of the microbiota. The synbiotic increased levels of Lactobacillus spp. and relative abundances of the genera Bifidobacterium, Collinsella, and Eubacterium while the genus Dialister was decreased (P

AB - Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n=18) were enrolled in a double-blinded, randomized, and placebo-controlled cross-over study and received synbiotic [Lactobacillus acidophilus NCFM (10(9)CFU) and cellobiose (5g)] or placebo daily for 3weeks. Fecal samples were collected and lactobacilli numbers were quantified by qPCR. Furthermore, 454 tag-encoded amplicon pyrosequencing was used to monitor the effect of synbiotic on the composition of the microbiota. The synbiotic increased levels of Lactobacillus spp. and relative abundances of the genera Bifidobacterium, Collinsella, and Eubacterium while the genus Dialister was decreased (P

KW - MICROBIOLOGY

KW - IRRITABLE-BOWEL-SYNDROME

KW - FECAL MICROBIOTA

KW - METABOLIC-ACTIVITY

KW - HEALTHY-VOLUNTEERS

KW - RHAMNOSUS KY-3

KW - IN-VITRO

KW - FERMENTATION

KW - SUPPLEMENTATION

KW - INTERVENTION

KW - CONSUMPTION

KW - synbiotics

KW - microbial community sequencing

KW - branched-chain fatty acids

KW - Lactobacillus

KW - Bifidobacterium

KW - human trial

U2 - 10.1111/1574-6941.12397

DO - 10.1111/1574-6941.12397

M3 - Journal article

C2 - 25098489

SN - 0168-6496

VL - 90

SP - 225

EP - 236

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

IS - 1

ER -

van Zanten GC, Krych L, Roytio H, Forssten S, Lahtinen SJ, Abu Al-Soud W et al. Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial. F E M S Microbiology Ecology. 2014;90(1):225-236. doi: 10.1111/1574-6941.12397