Swine plasma immunoglobulins for prevention and treatment of post-weaning diarrhoea: Optimizing stability towards gut conditions

Chris Juul Hedegaard, Anne-Sofie Ballegaard, Nanna Røjel, Marie Bendix Hansen, Bodil Kjær Lindved, Kirsten Bisgaard Frantzen, Lars Erik Larsen, Allan Lihme, Peter M. H. Heegaard

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    152 Downloads (Pure)

    Abstract

    Brief description of research area:
    A common problem in swine production is diarrhoea in newly weaned piglets, and huge quantities of antibiotics go to treat post-weaning diarrhoeas in piglets. The use of antibiotics can lead to the development of multi- and fully resistant bacteria, which consequently pose a great threat to human health. Therefore, sustainable alternatives for treating post-weaning diarrhoea without using antibiotics are in demand. Swine that are old (and big) enough for slaughter have during their upbringing been challenges by many different pathogens and thus have developed immunity towards these pathogens, which include pathogen-specific immunoglobulins (antibodies). We hypothesis that by harvesting natural immunoglobulins from porcine blood plasma, a waste product from swine slaughter, and feeding these immunoglobulins to the piglets this can subsequently (by passive immunisation) prevent and treat post-weaning diarrhoea.

    Our challenge is to find a suitable method for stabilising the immunoglobulins for oral provision in order for the immunoglobulins to pass as unharmed as possible through the digestive system and still retaining their anti-pathogenic properties.

    What we know:
    It is possible to multimerise immunoglobulins, which results in an advantage when binding to their respective antigens in comparison to the non-multimerised immunoglobulins, but too high degree of multimerisation abates immunoglobulin reactivity. Unfortunately, a preliminary study showed that multimerisation destabilises the immunglobulins. On the other hand, proteolytical resistance correlates with increased immunoglobulin concentration.

    What we need:
    To investigate the effect of increasing the concentration of multimerised immunoglobulins on proteolytical resistance.

    To investigate multimerised immunoglobulins’ ability in inhibiting microbial (E. coli) adhesion on relevant matrices, such intestinal villi and/or intestinal cell lines.

    A toxicological study on (if any) adverse side effects occurs when enteral providing immunoglobulins to piglets.
    Original languageEnglish
    Publication date2013
    Number of pages1
    Publication statusPublished - 2013
    Event10th Workshop in Protein.DTU - Technical University of Denmark, Kgs. Lyngby, Denmark
    Duration: 27 Nov 201327 Nov 2013
    http://www.bio.dtu.dk/english/Research/Research-groups/Enzyme-and-Protein-Chemistry/10-Workshop-in-Protein-DTU

    Workshop

    Workshop10th Workshop in Protein.DTU
    LocationTechnical University of Denmark
    CountryDenmark
    CityKgs. Lyngby
    Period27/11/201327/11/2013
    Internet address

    Activities

    • 1 Participating in or organising workshops, courses, seminars etc.

    10th Workshop in Protein.DTU

    Chris Juul Hedegaard (Participant)

    27 Nov 2013

    Activity: Attending an eventParticipating in or organising workshops, courses, seminars etc.

    File

    Cite this

    Hedegaard, C. J., Ballegaard, A-S., Røjel, N., Hansen, M. B., Lindved, B. K., Frantzen, K. B., Larsen, L. E., Lihme, A., & Heegaard, P. M. H. (2013). Swine plasma immunoglobulins for prevention and treatment of post-weaning diarrhoea: Optimizing stability towards gut conditions. Abstract from 10th Workshop in Protein.DTU, Kgs. Lyngby, Denmark.