Survey of inorganic arsenic in marine animals and marine certified reference materials by anion exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry

Jens Jørgen Sloth, Erik Huusfeldt Larsen, Kåre Julshamn

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A method for the determination of inorganic arsenic in seafood samples using high-performance liquid chromatography-inductively coupled plasma mass spectrometry is described. The principle of the method relied on microwave-assisted alkaline dissolution of the sample, which at the same time oxidized arsenite [As(Ill)] to arsenate [As(V)], whereby inorganic arsenic could be determined as the single species As(V). Anion exchange chromatography using isocratic elution with aqueous ammonium carbonate as the mobile phase was used for the separation of As(V) from other coextracted organoarsenic compounds, including arsenobetaine. The stability of organoarsenic compounds during the sample pretreatment was investigated, and no degradation/conversion to inorganic arsenic was detected. The method was employed for the determination of inorganic arsenic in a variety of seafood samples including fish, crustaceans, bivalves, and marine mammals as well as a range of marine certified reference materials, and the results were compared to values published in the literature. For fish and marine mammals, the results were in most cases below the limit of detection. For other sample types, inorganic arsenic concentrations up to 0.060 mg kg(-1) were found. In all samples, the inorganic arsenic content constituted less than 1% of the total arsenic content.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number15
Pages (from-to)6011-6018
ISSN0021-8561
DOIs
Publication statusPublished - 2005

Keywords

  • inorganic arsenic
  • alkaline dissolution
  • HPLC-ICPMS
  • seafood samples
  • certified reference materials

Cite this

@article{4871904d4f294d439d2c7112bf32be76,
title = "Survey of inorganic arsenic in marine animals and marine certified reference materials by anion exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry",
abstract = "A method for the determination of inorganic arsenic in seafood samples using high-performance liquid chromatography-inductively coupled plasma mass spectrometry is described. The principle of the method relied on microwave-assisted alkaline dissolution of the sample, which at the same time oxidized arsenite [As(Ill)] to arsenate [As(V)], whereby inorganic arsenic could be determined as the single species As(V). Anion exchange chromatography using isocratic elution with aqueous ammonium carbonate as the mobile phase was used for the separation of As(V) from other coextracted organoarsenic compounds, including arsenobetaine. The stability of organoarsenic compounds during the sample pretreatment was investigated, and no degradation/conversion to inorganic arsenic was detected. The method was employed for the determination of inorganic arsenic in a variety of seafood samples including fish, crustaceans, bivalves, and marine mammals as well as a range of marine certified reference materials, and the results were compared to values published in the literature. For fish and marine mammals, the results were in most cases below the limit of detection. For other sample types, inorganic arsenic concentrations up to 0.060 mg kg(-1) were found. In all samples, the inorganic arsenic content constituted less than 1{\%} of the total arsenic content.",
keywords = "inorganic arsenic, alkaline dissolution, HPLC-ICPMS, seafood samples, certified reference materials",
author = "Sloth, {Jens J{\o}rgen} and Larsen, {Erik Huusfeldt} and K{\aa}re Julshamn",
year = "2005",
doi = "10.1021/jf047950e",
language = "English",
volume = "53",
pages = "6011--6018",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "15",

}

Survey of inorganic arsenic in marine animals and marine certified reference materials by anion exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry. / Sloth, Jens Jørgen; Larsen, Erik Huusfeldt; Julshamn, Kåre.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 15, 2005, p. 6011-6018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Survey of inorganic arsenic in marine animals and marine certified reference materials by anion exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry

AU - Sloth, Jens Jørgen

AU - Larsen, Erik Huusfeldt

AU - Julshamn, Kåre

PY - 2005

Y1 - 2005

N2 - A method for the determination of inorganic arsenic in seafood samples using high-performance liquid chromatography-inductively coupled plasma mass spectrometry is described. The principle of the method relied on microwave-assisted alkaline dissolution of the sample, which at the same time oxidized arsenite [As(Ill)] to arsenate [As(V)], whereby inorganic arsenic could be determined as the single species As(V). Anion exchange chromatography using isocratic elution with aqueous ammonium carbonate as the mobile phase was used for the separation of As(V) from other coextracted organoarsenic compounds, including arsenobetaine. The stability of organoarsenic compounds during the sample pretreatment was investigated, and no degradation/conversion to inorganic arsenic was detected. The method was employed for the determination of inorganic arsenic in a variety of seafood samples including fish, crustaceans, bivalves, and marine mammals as well as a range of marine certified reference materials, and the results were compared to values published in the literature. For fish and marine mammals, the results were in most cases below the limit of detection. For other sample types, inorganic arsenic concentrations up to 0.060 mg kg(-1) were found. In all samples, the inorganic arsenic content constituted less than 1% of the total arsenic content.

AB - A method for the determination of inorganic arsenic in seafood samples using high-performance liquid chromatography-inductively coupled plasma mass spectrometry is described. The principle of the method relied on microwave-assisted alkaline dissolution of the sample, which at the same time oxidized arsenite [As(Ill)] to arsenate [As(V)], whereby inorganic arsenic could be determined as the single species As(V). Anion exchange chromatography using isocratic elution with aqueous ammonium carbonate as the mobile phase was used for the separation of As(V) from other coextracted organoarsenic compounds, including arsenobetaine. The stability of organoarsenic compounds during the sample pretreatment was investigated, and no degradation/conversion to inorganic arsenic was detected. The method was employed for the determination of inorganic arsenic in a variety of seafood samples including fish, crustaceans, bivalves, and marine mammals as well as a range of marine certified reference materials, and the results were compared to values published in the literature. For fish and marine mammals, the results were in most cases below the limit of detection. For other sample types, inorganic arsenic concentrations up to 0.060 mg kg(-1) were found. In all samples, the inorganic arsenic content constituted less than 1% of the total arsenic content.

KW - inorganic arsenic

KW - alkaline dissolution

KW - HPLC-ICPMS

KW - seafood samples

KW - certified reference materials

U2 - 10.1021/jf047950e

DO - 10.1021/jf047950e

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