Surface Plasmon Resonance Analysis for Quantifying Protein-Carbohydrate Interactions

Marie Sofie Møller, Darrell W Cockburn, Casper Wilkens

Research output: Chapter in Book/Report/Conference proceedingBook chapterEducationpeer-review

Abstract

During the past two decades, surface plasmon resonance (SPR) analysis has emerged as an important tool for studying protein-carbohydrate interactions, with several commercial instruments available. Binding affinities in the nM to mM range can be determined; however, there are pitfalls that require careful experimental design to avoid. Here we give an overview of each step in the SPR analysis from immobilization to data analysis, providing key points of consideration that will allow practitioners to achieve reliable and reproducible results.
Original languageEnglish
Title of host publicationCarbohydrate-Protein Interactions : Methods and Protocols
EditorsD. Wade Abbott, Wesley F. Zandberg
Number of pages10
Volume2657
PublisherSpringer
Publication date2023
Pages141-150
Chapter10
ISBN (Print)978-1-0716-3150-8, 978-1-0716-3153-9
ISBN (Electronic)978-1-0716-3151-5
DOIs
Publication statusPublished - 2023
SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Enzyme
  • Protein-carbonhydrate interactions
  • Surface plasmon resonance
  • Carbohydrate-binding module
  • Carbohydrate-active enzyme
  • Biosensor

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