TY - JOUR
T1 - Surface Modifications of Support Partitions for Stabilizing Biomimetic Membrane Arrays
AU - Perry, Mark
AU - Hansen, Jesper Schmidt
AU - Jensen, Karin Bagger Stibius
AU - Vissing, Thomas
AU - Pszon-Bartosz, Kamila Justyna
AU - Rein, Christian
AU - Eshtehardi, Bahram
AU - Benter, Maike
AU - Helix Nielsen, Claus
PY - 2011
Y1 - 2011
N2 - Black lipid membrane (BLM) formation across apertures in an ethylene tetra-fluoroethylene (ETFE) partition
separating two aqueous compartments is an established technique for the creation of biomimetic membranes.
Recently multi-aperture BLM arrays have attracted interest and in order to increase BLM array stability we studied
the effect of covalently modifying the partition substrate using surface plasma polymerization with hydrophobic
n-hexene, 1-decene and hexamethyldisiloxane (HMDSO) as modification groups. Average lifetimes across singlesided
HMDSO modified partitions or using 1-decene modified partitions were similar and significantly lower than
for arrays formed using untreated ETFE partitions. For single side n-hexene modification average membrane
array lifetimes were not significantly changed compared to untreated ETFE. Double-sided n-hexene modification
greatly improved average membrane array lifetimes compared to membrane arrays formed across untreated ETFE
partitions. n-hexene modifications resulted in BLM membrane arrays which over time developed significantly lower
conductance (Gm) and higher capacitance (Cm) values compared to the other membranes with the strongest effect
for double sided modification. n-hexene modification is evident as a change in surface energy whereas the surface
roughness does not change significantly. The concomitant low Gm and high Cm values for BLM arrays formed using
double-sided n-hexene modification enable transmembrane ionic current recordings with a high signal-to-noise (s/n)
ratio. We demonstratesd this by reconstituting gA and α-hemolysin (α-HL) into BLM arrays. The improvement in
membrane array lifetime and s/n ratio demonstrates that surface plasma polymerization of the supporting partition
can be used to increase the stability of biomimetic membrane arrays.
AB - Black lipid membrane (BLM) formation across apertures in an ethylene tetra-fluoroethylene (ETFE) partition
separating two aqueous compartments is an established technique for the creation of biomimetic membranes.
Recently multi-aperture BLM arrays have attracted interest and in order to increase BLM array stability we studied
the effect of covalently modifying the partition substrate using surface plasma polymerization with hydrophobic
n-hexene, 1-decene and hexamethyldisiloxane (HMDSO) as modification groups. Average lifetimes across singlesided
HMDSO modified partitions or using 1-decene modified partitions were similar and significantly lower than
for arrays formed using untreated ETFE partitions. For single side n-hexene modification average membrane
array lifetimes were not significantly changed compared to untreated ETFE. Double-sided n-hexene modification
greatly improved average membrane array lifetimes compared to membrane arrays formed across untreated ETFE
partitions. n-hexene modifications resulted in BLM membrane arrays which over time developed significantly lower
conductance (Gm) and higher capacitance (Cm) values compared to the other membranes with the strongest effect
for double sided modification. n-hexene modification is evident as a change in surface energy whereas the surface
roughness does not change significantly. The concomitant low Gm and high Cm values for BLM arrays formed using
double-sided n-hexene modification enable transmembrane ionic current recordings with a high signal-to-noise (s/n)
ratio. We demonstratesd this by reconstituting gA and α-hemolysin (α-HL) into BLM arrays. The improvement in
membrane array lifetime and s/n ratio demonstrates that surface plasma polymerization of the supporting partition
can be used to increase the stability of biomimetic membrane arrays.
U2 - 10.4172/2155-9589.S1-001
DO - 10.4172/2155-9589.S1-001
M3 - Journal article
SN - 2155-9589
JO - Journal of Membrane Science & Technology
JF - Journal of Membrane Science & Technology
IS - S: 1
ER -