Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications

Cuong Cao; Sam W. Birtwell; Jonas Høgberg; Anders Wolff; Hywel Morgan; Dang Duong Bang

Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications

Cuong Cao
, Sam W. Birtwell
, Jonas Høgberg
, Anders Wolff
, Hywel Morgan
, Dang Duong Bang

University of Southampton

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

This paper reports a surface modification of epoxy-based negative photoresist SU-8 for reducing its autofluorescence while enhancing its biofunctionality. By covalently depositing a thin layer of 20 nm Au nanoparticles (AuNPs) onto the SU-8 surface, we found that the AuNPs-coated SU-8 surface is much less fluorescent than the untreated SU-8. Moreover, DNA probes can easily be immobilized on the Au surface and are thermally stable over a wide range of temperature. These improvements will benefit bioanalytical applications such as DNA hybridization and solid-phase PCR (SP-PCR).

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Publication date	2011
Pages	1161-1163
ISBN (Print)	978-0-9798064-4-5
Publication status	Published - 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 Conference number: 15 https://microtasconferences.org/microtas2011/

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number	15
Country/Territory	United States
City	Seattle
Period	02/10/2011 → 06/10/2011
Internet address	https://microtasconferences.org/microtas2011/

Keywords

DNA Detection
SU-8
Gold Nanoparticles
Autofluorescence,
Photoresist

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@inproceedings{4dce3a9ed1564b739faab78439a2b75c,

title = "Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications",

abstract = "This paper reports a surface modification of epoxy-based negative photoresist SU-8 for reducing its autofluorescence while enhancing its biofunctionality. By covalently depositing a thin layer of 20 nm Au nanoparticles (AuNPs) onto the SU-8 surface, we found that the AuNPs-coated SU-8 surface is much less fluorescent than the untreated SU-8. Moreover, DNA probes can easily be immobilized on the Au surface and are thermally stable over a wide range of temperature. These improvements will benefit bioanalytical applications such as DNA hybridization and solid-phase PCR (SP-PCR).",

keywords = "DNA Detection, SU-8, Gold Nanoparticles, Autofluorescence,, Photoresist",

author = "Cuong Cao and Birtwell, \{Sam W.\} and Jonas H{\o}gberg and Anders Wolff and Hywel Morgan and Bang, \{Dang Duong\}",

year = "2011",

language = "English",

isbn = "978-0-9798064-4-5",

pages = "1161--1163",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

url = "https://microtasconferences.org/microtas2011/",

}

Cao, C, Birtwell, SW, Høgberg, J, Wolff, A, Morgan, H & Bang, DD 2011, Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1161-1163, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Seattle, Washington, United States, 02/10/2011.

Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications. / Cao, Cuong; Birtwell, Sam W.; Høgberg, Jonas et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2011. p. 1161-1163.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications

AU - Cao, Cuong

AU - Birtwell, Sam W.

AU - Høgberg, Jonas

AU - Wolff, Anders

AU - Morgan, Hywel

AU - Bang, Dang Duong

N1 - Conference code: 15

PY - 2011

Y1 - 2011

N2 - This paper reports a surface modification of epoxy-based negative photoresist SU-8 for reducing its autofluorescence while enhancing its biofunctionality. By covalently depositing a thin layer of 20 nm Au nanoparticles (AuNPs) onto the SU-8 surface, we found that the AuNPs-coated SU-8 surface is much less fluorescent than the untreated SU-8. Moreover, DNA probes can easily be immobilized on the Au surface and are thermally stable over a wide range of temperature. These improvements will benefit bioanalytical applications such as DNA hybridization and solid-phase PCR (SP-PCR).

AB - This paper reports a surface modification of epoxy-based negative photoresist SU-8 for reducing its autofluorescence while enhancing its biofunctionality. By covalently depositing a thin layer of 20 nm Au nanoparticles (AuNPs) onto the SU-8 surface, we found that the AuNPs-coated SU-8 surface is much less fluorescent than the untreated SU-8. Moreover, DNA probes can easily be immobilized on the Au surface and are thermally stable over a wide range of temperature. These improvements will benefit bioanalytical applications such as DNA hybridization and solid-phase PCR (SP-PCR).

KW - DNA Detection

KW - SU-8

KW - Gold Nanoparticles

KW - Autofluorescence,

KW - Photoresist

M3 - Article in proceedings

SN - 978-0-9798064-4-5

SP - 1161

EP - 1163

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 2 October 2011 through 6 October 2011

ER -

Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications

Abstract

Conference

Keywords

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