Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices

Research output: Contribution to journalJournal article – Annual report year: 2013Researchpeer-review

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Thiol-ene polymers possess physical, optical, and chemical characteristics thatmake them
ideal substrates for the fabrication of optofluidic devices. In this work, thiol-ene polymers
are used to simultaneously create microfluidic channels and optical waveguides in
one simple moulding step. The reactive functional groups present at the surface of the
thiol-ene polymer are subsequently used for the rapid, one step, site-specific functionalization
of the waveguide with biological recognition molecules. It was found that while
the bulk properties and chemical surface properties of thiol-ene materials vary considerably
with variations in stoichiometric composition, their optical properties remain mostly
unchanged with an average refractive index value of 1.566 ± 0.008 for thiol-ene substrates
encompassing a range from 150% excess ene to 90% excess thiol. Microfluidic chips featuring
thiol-ene waveguides were fabricated from 40% excess thiol thiol-ene to ensure the
presence of thiol functional groups at the surface of the waveguide. Biotin alkyne was
photografted at specific locations using a photomask, directly at the interface between the
microfluidic channel and the thiol-ene waveguide prior to conjugation with fluorescently
labeled streptavidin. Fluorescence excitation was achieved by launching light through the
thiol-ene waveguide, revealing bright fluorescent patterns along the channel/waveguide
interface
Original languageEnglish
JournalElectrophoresis
Volume35
Issue number2-3
Pages (from-to)282-288
ISSN0173-0835
DOIs
Publication statusPublished - 2013
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Biosensing, Fluorescence, Microfluidics, Surface functionalization, Thiol-ene

ID: 59237105