Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices

Nikolaj Agentoft Feidenhans'l, Josiane P. Lafleur, Thomas Glasdam Jensen, Jörg Peter Kutter

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Thiol-ene polymers possess physical, optical, and chemical characteristics thatmake them
    ideal substrates for the fabrication of optofluidic devices. In this work, thiol-ene polymers
    are used to simultaneously create microfluidic channels and optical waveguides in
    one simple moulding step. The reactive functional groups present at the surface of the
    thiol-ene polymer are subsequently used for the rapid, one step, site-specific functionalization
    of the waveguide with biological recognition molecules. It was found that while
    the bulk properties and chemical surface properties of thiol-ene materials vary considerably
    with variations in stoichiometric composition, their optical properties remain mostly
    unchanged with an average refractive index value of 1.566 ± 0.008 for thiol-ene substrates
    encompassing a range from 150% excess ene to 90% excess thiol. Microfluidic chips featuring
    thiol-ene waveguides were fabricated from 40% excess thiol thiol-ene to ensure the
    presence of thiol functional groups at the surface of the waveguide. Biotin alkyne was
    photografted at specific locations using a photomask, directly at the interface between the
    microfluidic channel and the thiol-ene waveguide prior to conjugation with fluorescently
    labeled streptavidin. Fluorescence excitation was achieved by launching light through the
    thiol-ene waveguide, revealing bright fluorescent patterns along the channel/waveguide
    interface
    Original languageEnglish
    JournalElectrophoresis
    Volume35
    Issue number2-3
    Pages (from-to)282-288
    ISSN0173-0835
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Biosensing
    • Fluorescence
    • Microfluidics
    • Surface functionalization
    • Thiol-ene

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