Surface-enhanced Raman spectroscopic study of DNA and 6-mercapto-1-hexanol interactions using large area mapping

Kasper Bayer Frøhling, Tommy Sonne Alstrøm, Michael Bache, Michael Stenbæk Schmidt, Mikkel Nørgaard Schmidt, Jan Larsen, Mogens Havsteen Jakobsen, Anja Boisen

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Abstract

The emergence of 2D SERS substrates with large areas of hot spots has enabled data to be gathered at large scale. This work presents a statistical tool for analysing large amounts of SERS data by utilizing a peak-fitting model in a specific spectral range. By analysing the distributions of Raman intensities and peak positions it is possible to directly inspect the interplay between DNA and 6-mercapto-1-hexanol on gold covered nanopillars. It is demonstrated that optimised functionalization parameters can be extracted from the Raman spectra directly. Using the peak-fitting approach it is possible to avoid miss-interpretation of intensity histograms, where contamination might contribute with an enhanced background and not a peak.
Original languageEnglish
JournalVibrational Spectroscopy
Volume86
Pages (from-to)331-336
ISSN0924-2031
DOIs
Publication statusPublished - 2016

Keywords

  • SERS
  • Area mapping
  • DNA
  • 6-mercapto-1-hexanol
  • Thiol
  • Gold
  • Peak-fitting

Cite this

@article{573f2b3271034a44b5cf81d1f535db23,
title = "Surface-enhanced Raman spectroscopic study of DNA and 6-mercapto-1-hexanol interactions using large area mapping",
abstract = "The emergence of 2D SERS substrates with large areas of hot spots has enabled data to be gathered at large scale. This work presents a statistical tool for analysing large amounts of SERS data by utilizing a peak-fitting model in a specific spectral range. By analysing the distributions of Raman intensities and peak positions it is possible to directly inspect the interplay between DNA and 6-mercapto-1-hexanol on gold covered nanopillars. It is demonstrated that optimised functionalization parameters can be extracted from the Raman spectra directly. Using the peak-fitting approach it is possible to avoid miss-interpretation of intensity histograms, where contamination might contribute with an enhanced background and not a peak.",
keywords = "SERS, Area mapping, DNA, 6-mercapto-1-hexanol, Thiol, Gold, Peak-fitting",
author = "Fr{\o}hling, {Kasper Bayer} and Alstr{\o}m, {Tommy Sonne} and Michael Bache and Schmidt, {Michael Stenb{\ae}k} and Schmidt, {Mikkel N{\o}rgaard} and Jan Larsen and Jakobsen, {Mogens Havsteen} and Anja Boisen",
year = "2016",
doi = "10.1016/j.vibspec.2016.08.005",
language = "English",
volume = "86",
pages = "331--336",
journal = "Vibrational Spectroscopy",
issn = "0924-2031",
publisher = "Elsevier",

}

Surface-enhanced Raman spectroscopic study of DNA and 6-mercapto-1-hexanol interactions using large area mapping. / Frøhling, Kasper Bayer; Alstrøm, Tommy Sonne; Bache, Michael; Schmidt, Michael Stenbæk; Schmidt, Mikkel Nørgaard; Larsen, Jan; Jakobsen, Mogens Havsteen; Boisen, Anja.

In: Vibrational Spectroscopy, Vol. 86, 2016, p. 331-336.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Surface-enhanced Raman spectroscopic study of DNA and 6-mercapto-1-hexanol interactions using large area mapping

AU - Frøhling, Kasper Bayer

AU - Alstrøm, Tommy Sonne

AU - Bache, Michael

AU - Schmidt, Michael Stenbæk

AU - Schmidt, Mikkel Nørgaard

AU - Larsen, Jan

AU - Jakobsen, Mogens Havsteen

AU - Boisen, Anja

PY - 2016

Y1 - 2016

N2 - The emergence of 2D SERS substrates with large areas of hot spots has enabled data to be gathered at large scale. This work presents a statistical tool for analysing large amounts of SERS data by utilizing a peak-fitting model in a specific spectral range. By analysing the distributions of Raman intensities and peak positions it is possible to directly inspect the interplay between DNA and 6-mercapto-1-hexanol on gold covered nanopillars. It is demonstrated that optimised functionalization parameters can be extracted from the Raman spectra directly. Using the peak-fitting approach it is possible to avoid miss-interpretation of intensity histograms, where contamination might contribute with an enhanced background and not a peak.

AB - The emergence of 2D SERS substrates with large areas of hot spots has enabled data to be gathered at large scale. This work presents a statistical tool for analysing large amounts of SERS data by utilizing a peak-fitting model in a specific spectral range. By analysing the distributions of Raman intensities and peak positions it is possible to directly inspect the interplay between DNA and 6-mercapto-1-hexanol on gold covered nanopillars. It is demonstrated that optimised functionalization parameters can be extracted from the Raman spectra directly. Using the peak-fitting approach it is possible to avoid miss-interpretation of intensity histograms, where contamination might contribute with an enhanced background and not a peak.

KW - SERS

KW - Area mapping

KW - DNA

KW - 6-mercapto-1-hexanol

KW - Thiol

KW - Gold

KW - Peak-fitting

U2 - 10.1016/j.vibspec.2016.08.005

DO - 10.1016/j.vibspec.2016.08.005

M3 - Journal article

VL - 86

SP - 331

EP - 336

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

SN - 0924-2031

ER -