Surface-enhanced Raman spectroscopic study of DNA and 6-mercapto-1-hexanol interactions using large area mapping

Kasper Bayer Frøhling, Tommy Sonne Alstrøm, Michael Bache, Michael Stenbæk Schmidt, Mikkel Nørgaard Schmidt, Jan Larsen, Mogens Havsteen Jakobsen, Anja Boisen

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The emergence of 2D SERS substrates with large areas of hot spots has enabled data to be gathered at large scale. This work presents a statistical tool for analysing large amounts of SERS data by utilizing a peak-fitting model in a specific spectral range. By analysing the distributions of Raman intensities and peak positions it is possible to directly inspect the interplay between DNA and 6-mercapto-1-hexanol on gold covered nanopillars. It is demonstrated that optimised functionalization parameters can be extracted from the Raman spectra directly. Using the peak-fitting approach it is possible to avoid miss-interpretation of intensity histograms, where contamination might contribute with an enhanced background and not a peak.
Original languageEnglish
JournalVibrational Spectroscopy
Pages (from-to)331-336
Publication statusPublished - 2016


  • SERS
  • Area mapping
  • DNA
  • 6-mercapto-1-hexanol
  • Thiol
  • Gold
  • Peak-fitting

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