Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

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@article{cb08fdeb99f64983a5ef9cf2ecd73eab,
title = "Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray",
abstract = "Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.",
keywords = "Microarray, Silica, Magnetic bead, Streptavidin, Biotin",
author = "Skottrup, {Peter Durand} and Hansen, {Mikkel Fougt} and Moresco, {Jacob Lange} and Maria Deryabina and Svendsen, {Winnie Edith} and Jakobsen, {Mogens Havsteen} and Martin Dufva",
year = "2010",
doi = "10.1016/j.actbio.2010.04.019",
language = "English",
volume = "6",
pages = "3936--3946",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

AU - Skottrup, Peter Durand

AU - Hansen, Mikkel Fougt

AU - Moresco, Jacob Lange

AU - Deryabina, Maria

AU - Svendsen, Winnie Edith

AU - Jakobsen, Mogens Havsteen

AU - Dufva, Martin

PY - 2010

Y1 - 2010

N2 - Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.

AB - Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.

KW - Microarray

KW - Silica

KW - Magnetic bead

KW - Streptavidin

KW - Biotin

U2 - 10.1016/j.actbio.2010.04.019

DO - 10.1016/j.actbio.2010.04.019

M3 - Journal article

VL - 6

SP - 3936

EP - 3946

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 10

ER -