Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

Peter Durand Skottrup, Mikkel Fougt Hansen, Jacob Lange Moresco, Maria Deryabina, Winnie Edith Svendsen, Mogens Havsteen Jakobsen, Martin Dufva

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
    Original languageEnglish
    JournalActa Biomaterialia
    Volume6
    Issue number10
    Pages (from-to)3936-3946
    ISSN1742-7061
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Microarray
    • Silica
    • Magnetic bead
    • Streptavidin
    • Biotin

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