Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion

Sofie Bech Andersen, Carlos Armando Villagómez Hoyos, Iman Taghavi, Fredrik Granberg, Kristoffer Lindskov Hansen, Charlotte Mehlin Sørensen, Jørgen Arendt Jensen, Michael Bachmann Nielsen

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

225 Downloads (Pure)

Abstract

In vivo visualization of the microvasculature is feasible with super-resolution ultrasound imaging (SRI), but the method needs more affirmative data before clinical use. The kidneys have a rich vasculature, and microvascular dysfunction decreases the kidney function. Therefore, detection of subtle renal microvascular changes could benefit patients with renal disease. We hypothesized that our SRI setup can visualize the microvascular network of two healthy rat kidneys and subsequently demonstrate
microvascular flow changes immediately after ischemiareperfusion. The left kidney of two male Sprague-Dawley rats was scanned during laparotomy using a customized BK5000 scanner, an X18L5s transducer, and interleaved contrast and B-mode sequences with focused beam transmission. Images were acquired over 10 min using SonoVue (1:10) as the contrast agent. After a baseline scan, one rat had the renal vein clamped, while the other rat had the renal artery clamped, both for 45 min. The kidneys were rescanned immediately after clamp release and after 60 min of reperfusion. Motion correction was applied before microbubble (MB) detection. The characteristic renal microvascular structure was visualized with anatomical distinction between the dense cortical vascular network and the straight vessels of the medulla. Immediately after vein clamp release, almost no MBs perfused the medullary vessels. After 60 min of reperfusion, MBs refilled the renal vascular bed, but with a slower velocity compared with the baseline scan. The results after artery clamping were subtler, with more MBs perfusing the medulla immediately after clamp release compared with the vein clamping. The results imply that this SRI setup can evaluate different stages of ischemic kidney disease in rats as it can visualize the entire renal vascular bed and differentiate the pattern of reperfusion in two types of ischemic injury.
Original languageEnglish
Title of host publicationProceedings of 2019 IEEE International Ultrasonics Symposium
PublisherIEEE
Publication date2019
Pages1169-1172
ISBN (Electronic)978-1-7281-4596-9
DOIs
Publication statusPublished - 2019
Event2019 IEEE International Ultrasonics Symposium - SEC Glasgow, Glasgow, United Kingdom
Duration: 6 Oct 20199 Oct 2019
http://attend.ieee.org/ius-2019/

Conference

Conference2019 IEEE International Ultrasonics Symposium
LocationSEC Glasgow
Country/TerritoryUnited Kingdom
CityGlasgow
Period06/10/201909/10/2019
Internet address

Fingerprint

Dive into the research topics of 'Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion'. Together they form a unique fingerprint.

Cite this