1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

Agnes Beenfeldt Petersen; Idd Andrea Christensen; Mette E. Rønne; Emil G. P. Stender; David Teze; Birte Svensson; Finn Lillelund Aachmann

doi:10.1007/s12104-022-10102-6

¹H, ¹³C, ¹⁵N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

Agnes Beenfeldt Petersen, Idd Andrea Christensen, Mette E. Rønne, Emil G. P. Stender, David Teze, Birte Svensson, Finn Lillelund Aachmann^*

^*Corresponding author for this work

Norwegian University of Science and Technology

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Abstract

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the ¹H, ¹³C, and ¹⁵N NMR chemical shift assignment for BeKdgF is reported.

Original language	English
Journal	Biomolecular N M R Assignments
Volume	16
Pages (from-to)	343-347
Number of pages	5
ISSN	1874-2718
DOIs	https://doi.org/10.1007/s12104-022-10102-6
Publication status	Published - 2022

Keywords

KdgF
Alginate degradation
4-deoxy-L-erythro-5-hexoseulose (DEH)
Human gut microbiota

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title = "1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii",

abstract = "To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the 1H, 13C, and 15N NMR chemical shift assignment for BeKdgF is reported.",

keywords = "KdgF, Alginate degradation, 4-deoxy-L-erythro-5-hexoseulose (DEH), Human gut microbiota",

author = "Petersen, {Agnes Beenfeldt} and Christensen, {Idd Andrea} and R{\o}nne, {Mette E.} and Stender, {Emil G. P.} and David Teze and Birte Svensson and Aachmann, {Finn Lillelund}",

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doi = "10.1007/s12104-022-10102-6",

language = "English",

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T1 - 1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

AU - Petersen, Agnes Beenfeldt

AU - Christensen, Idd Andrea

AU - Rønne, Mette E.

AU - Stender, Emil G. P.

AU - Teze, David

AU - Svensson, Birte

AU - Aachmann, Finn Lillelund

PY - 2022

Y1 - 2022

N2 - To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the 1H, 13C, and 15N NMR chemical shift assignment for BeKdgF is reported.

AB - To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the 1H, 13C, and 15N NMR chemical shift assignment for BeKdgF is reported.

KW - KdgF

KW - Alginate degradation

KW - 4-deoxy-L-erythro-5-hexoseulose (DEH)

KW - Human gut microbiota

U2 - 10.1007/s12104-022-10102-6

DO - 10.1007/s12104-022-10102-6

M3 - Journal article

C2 - 36042150

SN - 1874-2718

VL - 16

SP - 343

EP - 347

JO - Biomolecular N M R Assignments

JF - Biomolecular N M R Assignments

ER -