1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis

Eva Madland, Yoshihito Kitaoku, Gerd Inger Sætrom, Maria Louise Leth, Morten Ejby, Maher Abou Hachem, Finn Lillelund Aachmann*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The N-terminal domain (residues 28–165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).
Original languageEnglish
JournalBiomolecular N M R Assignments
Volume13
Issue number1
Pages (from-to)55-58
Number of pages4
ISSN1874-2718
DOIs
Publication statusPublished - 2019

Keywords

  • Carbohydrate binding module (CBM)
  • Gut microbiota
  • Roseburia intestinalis
  • Xylan binding module
  • Xylanase

Cite this

Madland, Eva ; Kitaoku, Yoshihito ; Sætrom, Gerd Inger ; Leth, Maria Louise ; Ejby, Morten ; Abou Hachem, Maher ; Aachmann, Finn Lillelund. / 1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis. In: Biomolecular N M R Assignments. 2019 ; Vol. 13, No. 1. pp. 55-58.
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title = "1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis",
abstract = "The N-terminal domain (residues 28{\^a}€“165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).",
keywords = "Carbohydrate binding module (CBM), Gut microbiota, Roseburia intestinalis, Xylan binding module, Xylanase",
author = "Eva Madland and Yoshihito Kitaoku and S{\ae}trom, {Gerd Inger} and Leth, {Maria Louise} and Morten Ejby and {Abou Hachem}, Maher and Aachmann, {Finn Lillelund}",
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doi = "10.1007/s12104-018-9850-3",
language = "English",
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1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis. / Madland, Eva; Kitaoku, Yoshihito; Sætrom, Gerd Inger; Leth, Maria Louise; Ejby, Morten; Abou Hachem, Maher ; Aachmann, Finn Lillelund.

In: Biomolecular N M R Assignments, Vol. 13, No. 1, 2019, p. 55-58.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - 1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis

AU - Madland, Eva

AU - Kitaoku, Yoshihito

AU - Sætrom, Gerd Inger

AU - Leth, Maria Louise

AU - Ejby, Morten

AU - Abou Hachem, Maher

AU - Aachmann, Finn Lillelund

PY - 2019

Y1 - 2019

N2 - The N-terminal domain (residues 28–165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).

AB - The N-terminal domain (residues 28–165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).

KW - Carbohydrate binding module (CBM)

KW - Gut microbiota

KW - Roseburia intestinalis

KW - Xylan binding module

KW - Xylanase

U2 - 10.1007/s12104-018-9850-3

DO - 10.1007/s12104-018-9850-3

M3 - Journal article

VL - 13

SP - 55

EP - 58

JO - Biomolecular N M R Assignments

JF - Biomolecular N M R Assignments

SN - 1874-2718

IS - 1

ER -