1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis

Eva Madland; Yoshihito Kitaoku; Gerd Inger Sætrom; Maria Louise Leth; Morten Ejby; Maher  Abou Hachem; Finn Lillelund Aachmann

doi:10.1007/s12104-018-9850-3

¹H, ¹³C and ¹⁵N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis

Eva Madland, Yoshihito Kitaoku, Gerd Inger Sætrom, Maria Louise Leth, Morten Ejby, Maher Abou Hachem, Finn Lillelund Aachmann^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The N-terminal domain (residues 28â€“165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report¹H, ¹³C and ¹⁵N NMR chemical shift assignments for this carbohydrate binding module (CBM).

Original language	English
Journal	Biomolecular N M R Assignments
Volume	13
Issue number	1
Pages (from-to)	55-58
Number of pages	4
ISSN	1874-2718
DOIs	https://doi.org/10.1007/s12104-018-9850-3
Publication status	Published - 2019

Keywords

Carbohydrate binding module (CBM)
Gut microbiota
Roseburia intestinalis
Xylan binding module
Xylanase

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10.1007/s12104-018-9850-3

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title = "1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis",

abstract = "The N-terminal domain (residues 28{\^a}€“165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).",

keywords = "Carbohydrate binding module (CBM), Gut microbiota, Roseburia intestinalis, Xylan binding module, Xylanase",

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T1 - 1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis

AU - Madland, Eva

AU - Kitaoku, Yoshihito

AU - Sætrom, Gerd Inger

AU - Leth, Maria Louise

AU - Ejby, Morten

AU - Abou Hachem, Maher

AU - Aachmann, Finn Lillelund

PY - 2019

Y1 - 2019

N2 - The N-terminal domain (residues 28â€“165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).

AB - The N-terminal domain (residues 28â€“165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and 15N NMR chemical shift assignments for this carbohydrate binding module (CBM).

KW - Carbohydrate binding module (CBM)

KW - Gut microbiota

KW - Roseburia intestinalis

KW - Xylan binding module

KW - Xylanase

U2 - 10.1007/s12104-018-9850-3

DO - 10.1007/s12104-018-9850-3

M3 - Journal article

C2 - 30244308

SN - 1874-2718

VL - 13

SP - 55

EP - 58

JO - Biomolecular N M R Assignments

JF - Biomolecular N M R Assignments

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ER -