SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

Sonia Silva, Veronika Altmannova, Nadine Eckert-Boulet, Peter Kolesar, Irene Gallina, Lisa Hang, Inn Chun, Milica Arneric, Xiaolan Zhao, Line Due Buron, Uffe Hasbro Mortensen, Lumir Krejci, Michael Lisby

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    Abstract

    Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR.
    Original languageEnglish
    JournalD N A Repair
    Volume42
    Pages (from-to)11-25
    Number of pages15
    ISSN1568-7864
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Homologous recombination
    • Rad52
    • Rad59
    • Srs2
    • Rad51
    • SUMOylation

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