Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies

A. Saalmuller, G. Kuebart, E. Hollemweguer, Z. Chen, Jens Nielsen, F. Zuckermann, K. Haverson

    Research output: Contribution to journalJournal articlepeer-review

    Abstract

    Fifty-seven monoclonal antibodies (mAb) selected after the first round analyses in the Third International Swine CD workshop for their possible reactivity with T-lymphocyte specific antigens were further analysed in a second round. As target cells for flow cytometric analyses served peripheral blood mononuclear cells, nylon-wool enriched T-lymphocytes, thymocytes, splenocytes, and lymphocytes derived from Peyer's patches. These second round analyses revealed 15 different data sets. Together with 22 pre-selected data sets from the first round analyses with the whole panel of monoclonal antibodies, 37 data sets were used for the clustering of the respective mAb. Using the LTDB4 program, 19 preliminary clusters could be defined. Two clusters (C3 and C7) with 4 mAb showed no labelling of resting T-lymphocytes. Seven clusters (CI, C2, C4, C5, C6, C11, and C12) contain mAb (in total: 16 mAb) directed against subsets of CD4(-)CD8(-) T-lymphocytes. These mAb seem to recognise antigens on porcine T-lymphocytes with T-cell receptor (TcR) gamma/delta chains. Three clusters (C8, C9, CIO, C13) seem to be artificial. They contain either mAb staining CD4(-)CD8(-) T-lymphocytes and low CD8+ cells (C8, C9), mAb with various reactivity (CIO) and mAb with known differences in their reactivity (C13). Cluster C14 contains 3 mAb against the CD4a-epitope, C15 describes mAb directed against porcine CD8c-epitope whereas mAb against CD8a and CD8b-epitopes grouped in C19. The mAb found in C16 seem to recognise CD45R. Cluster C17 is composed of different standards directed against CD2, CD3, CD5 and wCD6. Two additional mAb recognising the CD2a-epitope could be enclosed. CIS contains two mAb directed against SWC2.
    Original languageEnglish
    JournalVeterinary Immunology and Immunopathology
    Volume80
    Issue number1-2
    Pages (from-to)35-52
    ISSN0165-2427
    DOIs
    Publication statusPublished - 2001

    Keywords

    • monoclonal antibodies (mAb)
    • CD2
    • cluster of differentiation (CD) antigens
    • SWC2
    • CD4
    • CD3
    • CD8
    • pig

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