Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device

Helene Larsen*, Nicole Bakkegård Goecke, Charlotte Kristiane Hjulsager

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Reverse transcription real-time PCR (RT-qPCR) is one of several techniques used to determine the presence and level of infectious veterinary pathogens in diagnostic laboratories. Here we describe how automation of PCR reactions using integrated fluidic circuits (IFCs), an IFC controller MX and a Biomark HD instrument allows for the testing of 48 field samples with swine influenza for up to 48 different subtypes simultaneously in nanoliter volumes.
Original languageEnglish
Title of host publicationNucleic Acid Detection and Structural Investigations
EditorsKira Astakhova, Syeda Atia Bukhari
PublisherSpringer Science+Business Media
Publication date2020
Pages17-25
Chapter2
ISBN (Print)978-1-0716-0137-2
ISBN (Electronic)978-1-0716-0138-9
DOIs
Publication statusPublished - 2020
SeriesMethods in Molecular Biology
Number2063
ISSN1064-3745

Cite this

Larsen, H., Goecke, N. B., & Hjulsager, C. K. (2020). Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device. In K. Astakhova, & S. Atia Bukhari (Eds.), Nucleic Acid Detection and Structural Investigations (pp. 17-25). Springer Science+Business Media. Methods in Molecular Biology, No. 2063 https://doi.org/10.1007/978-1-0716-0138-9_2