Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device

Helene Larsen; Nicole Bakkegård Goecke; Charlotte Kristiane Hjulsager

doi:10.1007/978-1-0716-0138-9_2

Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device

Helene Larsen^*, Nicole Bakkegård Goecke, Charlotte Kristiane Hjulsager

^*Corresponding author for this work

Statens Serum Institut

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Reverse transcription real-time PCR (RT-qPCR) is one of several techniques used to determine the presence and level of infectious veterinary pathogens in diagnostic laboratories. Here we describe how automation of PCR reactions using integrated fluidic circuits (IFCs), an IFC controller MX and a Biomark HD instrument allows for the testing of 48 field samples with swine influenza for up to 48 different subtypes simultaneously in nanoliter volumes.

Original language	English
Title of host publication	Nucleic Acid Detection and Structural Investigations
Editors	Kira Astakhova, Syeda Atia Bukhari
Publisher	Springer Science+Business Media
Publication date	2020
Pages	17-25
Chapter	2
ISBN (Print)	978-1-0716-0137-2
ISBN (Electronic)	978-1-0716-0138-9
DOIs	https://doi.org/10.1007/978-1-0716-0138-9_2
Publication status	Published - 2020

Series	Methods in Molecular Biology
Number	2063
ISSN	1064-3745

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Larsen, Helene ; Goecke, Nicole Bakkegård ; Hjulsager, Charlotte Kristiane. / Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device. Nucleic Acid Detection and Structural Investigations. editor / Kira Astakhova ; Syeda Atia Bukhari. Springer Science+Business Media, 2020. pp. 17-25 (Methods in Molecular Biology; No. 2063).

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Larsen, H, Goecke, NB & Hjulsager, CK 2020, Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device. in K Astakhova & S Atia Bukhari (eds), Nucleic Acid Detection and Structural Investigations. Springer Science+Business Media, Methods in Molecular Biology, no. 2063, pp. 17-25. https://doi.org/10.1007/978-1-0716-0138-9_2

Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device. / Larsen, Helene; Goecke, Nicole Bakkegård; Hjulsager, Charlotte Kristiane.
Nucleic Acid Detection and Structural Investigations. ed. / Kira Astakhova; Syeda Atia Bukhari. Springer Science+Business Media, 2020. p. 17-25 (Methods in Molecular Biology; No. 2063).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device

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