Substrate supply for effective biocatalysis

P-Y Kim, DJ Pollard, John Woodley

Research output: Contribution to journalJournal articleResearchpeer-review


Using biocatalysis for some chemical synthesis steps has unique advantages such as achieving higher product selectivity under ambient process conditions. However, a common limitation with such systems is the inhibition or toxicity posed by the starting substrate as well as limited aqueous solubility in many cases. In this review, we discuss the supply of substrate to bioconversions. The delivery of substrate via an auxiliary, which may be water-miscible, or a second phase such as a water-immiscible organic solvent, adsorbing resin, or a gas, is examined through recent examples in the field. Finally, guidelines for experimental planning and process considerations are suggested to facilitate the choice of substrate delivery method and accelerate process development.
Original languageEnglish
JournalBiotechnology Progress
Issue number1
Pages (from-to)74-82
Publication statusPublished - 2007
Externally publishedYes

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