Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans

Troels Lillebaek, Anders Norman, Erik Michael Rasmussen, Rasmus Lykke Marvig, Dorte Bek Folkvardsen, Åse Bengaard Andersen, Lars Jelsbak

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    The genome of Mycobacterium tuberculosis (Mtb) of latently infected individuals may hold the key to understanding the processes that lead to reactivation and progression to clinical disease. We report here analysis of pairs of Mtb isolates from putative prolonged latent TB cases. We identified two confirmed cases, and used whole genome sequencing to investigate the mutational processes that occur over decades in latent Mtb. We found an estimated mutation rate between 0.2 and 0.3 over 33 years, suggesting that latent Mtb accumulates mutations at rates similar to observations from cases of active disease.
    Original languageEnglish
    JournalInternational Journal of Medical Microbiology
    Volume306
    Issue number7
    Pages (from-to)580-585
    Number of pages6
    ISSN1438-4221
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Latent infection
    • Within-host evolution
    • Mutation rate
    • Genome sequencing
    • Pathogen evolution

    Cite this

    Lillebaek, Troels ; Norman, Anders ; Rasmussen, Erik Michael ; Marvig, Rasmus Lykke ; Bek Folkvardsen, Dorte ; Bengaard Andersen, Åse ; Jelsbak, Lars. / Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans. In: International Journal of Medical Microbiology. 2016 ; Vol. 306, No. 7. pp. 580-585.
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    Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans. / Lillebaek, Troels; Norman, Anders; Rasmussen, Erik Michael; Marvig, Rasmus Lykke; Bek Folkvardsen, Dorte; Bengaard Andersen, Åse; Jelsbak, Lars.

    In: International Journal of Medical Microbiology, Vol. 306, No. 7, 2016, p. 580-585.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    AU - Lillebaek, Troels

    AU - Norman, Anders

    AU - Rasmussen, Erik Michael

    AU - Marvig, Rasmus Lykke

    AU - Bek Folkvardsen, Dorte

    AU - Bengaard Andersen, Åse

    AU - Jelsbak, Lars

    PY - 2016

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    AB - The genome of Mycobacterium tuberculosis (Mtb) of latently infected individuals may hold the key to understanding the processes that lead to reactivation and progression to clinical disease. We report here analysis of pairs of Mtb isolates from putative prolonged latent TB cases. We identified two confirmed cases, and used whole genome sequencing to investigate the mutational processes that occur over decades in latent Mtb. We found an estimated mutation rate between 0.2 and 0.3 over 33 years, suggesting that latent Mtb accumulates mutations at rates similar to observations from cases of active disease.

    KW - Latent infection

    KW - Within-host evolution

    KW - Mutation rate

    KW - Genome sequencing

    KW - Pathogen evolution

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