Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans

Troels Lillebaek; Anders Norman; Erik Michael Rasmussen; Rasmus Lykke Marvig; Dorte Bek Folkvardsen; Åse Bengaard Andersen; Lars Jelsbak

doi:10.1016/j.ijmm.2016.05.017

Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans

Troels Lillebaek, Anders Norman, Erik Michael Rasmussen, Rasmus Lykke Marvig, Dorte Bek Folkvardsen, Åse Bengaard Andersen, Lars Jelsbak

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The genome of Mycobacterium tuberculosis (Mtb) of latently infected individuals may hold the key to understanding the processes that lead to reactivation and progression to clinical disease. We report here analysis of pairs of Mtb isolates from putative prolonged latent TB cases. We identified two confirmed cases, and used whole genome sequencing to investigate the mutational processes that occur over decades in latent Mtb. We found an estimated mutation rate between 0.2 and 0.3 over 33 years, suggesting that latent Mtb accumulates mutations at rates similar to observations from cases of active disease.

Original language	English
Journal	International Journal of Medical Microbiology
Volume	306
Issue number	7
Pages (from-to)	580-585
Number of pages	6
ISSN	1438-4221
DOIs	https://doi.org/10.1016/j.ijmm.2016.05.017
Publication status	Published - 2016

Keywords

Latent infection
Within-host evolution
Mutation rate
Genome sequencing
Pathogen evolution

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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@article{231a96a108884b1a823d1e3e2d219022,

title = "Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans",

abstract = "The genome of Mycobacterium tuberculosis (Mtb) of latently infected individuals may hold the key to understanding the processes that lead to reactivation and progression to clinical disease. We report here analysis of pairs of Mtb isolates from putative prolonged latent TB cases. We identified two confirmed cases, and used whole genome sequencing to investigate the mutational processes that occur over decades in latent Mtb. We found an estimated mutation rate between 0.2 and 0.3 over 33 years, suggesting that latent Mtb accumulates mutations at rates similar to observations from cases of active disease.",

keywords = "Latent infection, Within-host evolution, Mutation rate, Genome sequencing, Pathogen evolution",

author = "Troels Lillebaek and Anders Norman and Rasmussen, {Erik Michael} and Marvig, {Rasmus Lykke} and {Bek Folkvardsen}, Dorte and {Bengaard Andersen}, {\AA}se and Lars Jelsbak",

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doi = "10.1016/j.ijmm.2016.05.017",

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Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans. / Lillebaek, Troels; Norman, Anders; Rasmussen, Erik Michael; Marvig, Rasmus Lykke; Bek Folkvardsen, Dorte; Bengaard Andersen, Åse; Jelsbak, Lars.

In: International Journal of Medical Microbiology, Vol. 306, No. 7, 2016, p. 580-585.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Rasmussen, Erik Michael

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AU - Bengaard Andersen, Åse

AU - Jelsbak, Lars

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KW - Latent infection

KW - Within-host evolution

KW - Mutation rate

KW - Genome sequencing

KW - Pathogen evolution

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