Subcellular localization of Aleutian mink disease parvovirus proteins and DNA during permissive infection of Crandell feline kidney cells

M.B. Oleksiewicz, F. Costello, M. Huhtanen, J.B. Wolfinbarger, Søren Alexandersen, M.E. Bloom

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Confocal microscopy allowed us to localize viral nonstructural (NS) and capsid (VP) proteins and DNA simultaneously in cells permissively infected with Aleutian mink disease parvovirus (ADV). Early after infection, NS proteins colocalized with viral DNA to form intranuclear inclusions, whereas VP proteins formed hollow intranuclear shells around the inclusions. Later, nuclei had irregular outlines and were virtually free of ADV products. In these cells, inclusions of viral DNA with or without associated NS protein were embedded in cytoplasmic VP protein. These findings implied that ADV replication within an infected cell is regulated spatially as well as temporally.
    Original languageEnglish
    JournalJournal of Virology
    Volume70
    Issue number5
    Pages (from-to)3242-3247
    ISSN0022-538X
    Publication statusPublished - 1996

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