Subacute oral toxicity investigation of selenium nanoparticles and selenite in rats

Niels Hadrup, Katrin Löschner, Karen Mandrup Egebjerg, Gitte Ravn-Haren, Henrik Lauritz Frandsen, Erik Huusfeldt Larsen, Henrik R. Lam, Alicja Mortensen*

*Corresponding author for this work

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Abstract

Selenium (Se) nanoparticles have been proposed as food supplements. However, the particle formulation may exert unexpected toxicity. The aim was therefore to compare toxicity of low doses of Se nanoparticles and the dissolved, ionized Se species selenite. Female rats were dosed orally for 28 d with either: 0.05, 0.5, or 4 mg Se/kg body weight (bw)/day as 20 nm Se nanoparticles or 0.05 or 0.5 mg Se/kg bw/day as sodium selenite. Male rats were dosed 4 mg Se/kg bw/day as Se nanoparticles. Body weight and clinical appearance were recorded throughout the experiment. At necropsy, blood samples were taken for hematological and clinical chemistry analyses; organ weights were recorded. At the high-dose of Se nanoparticles, overt toxicity occurred and the female animals had to be euthanized prematurely, whereas the male animals were reduced in dose. At all doses of Se nanoparticles and at 0.5 mg Se/kg bw/day as selenite, a lower body weight gain as compared to vehicle occurred. Relative liver weight was increased for both Se formulations at 0.5 mg Se/kg bw/day. Creatinine clearance and urinary pH were affected in some Se dosed groups. There were no effects among dosed groups on brain neurotransmitters or on hematological parameters compared with controls. There were no histological changes in the livers of animals exposed to Se nanoparticles or to selenite. Based on effects on body weight and liver weight, selenium nanoparticles and ionic Se exerted similar toxicity. This suggests that a nanoparticle-specific toxicity of Se did not occur.
Original languageEnglish
JournalDrug and Chemical Toxicology
Volume42
Issue number1
Pages (from-to)76-83
Number of pages8
ISSN0148-0545
DOIs
Publication statusPublished - 2019

Keywords

  • Selenium
  • Nanoparticle
  • Sodium selenite
  • Toxicity
  • Subacute

Cite this

Hadrup, Niels ; Löschner, Katrin ; Mandrup Egebjerg, Karen ; Ravn-Haren, Gitte ; Frandsen, Henrik Lauritz ; Larsen, Erik Huusfeldt ; Lam, Henrik R. ; Mortensen, Alicja. / Subacute oral toxicity investigation of selenium nanoparticles and selenite in rats. In: Drug and Chemical Toxicology. 2019 ; Vol. 42, No. 1. pp. 76-83.
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title = "Subacute oral toxicity investigation of selenium nanoparticles and selenite in rats",
abstract = "Selenium (Se) nanoparticles have been proposed as food supplements. However, the particle formulation may exert unexpected toxicity. The aim was therefore to compare toxicity of low doses of Se nanoparticles and the dissolved, ionized Se species selenite. Female rats were dosed orally for 28 d with either: 0.05, 0.5, or 4 mg Se/kg body weight (bw)/day as 20 nm Se nanoparticles or 0.05 or 0.5 mg Se/kg bw/day as sodium selenite. Male rats were dosed 4 mg Se/kg bw/day as Se nanoparticles. Body weight and clinical appearance were recorded throughout the experiment. At necropsy, blood samples were taken for hematological and clinical chemistry analyses; organ weights were recorded. At the high-dose of Se nanoparticles, overt toxicity occurred and the female animals had to be euthanized prematurely, whereas the male animals were reduced in dose. At all doses of Se nanoparticles and at 0.5 mg Se/kg bw/day as selenite, a lower body weight gain as compared to vehicle occurred. Relative liver weight was increased for both Se formulations at 0.5 mg Se/kg bw/day. Creatinine clearance and urinary pH were affected in some Se dosed groups. There were no effects among dosed groups on brain neurotransmitters or on hematological parameters compared with controls. There were no histological changes in the livers of animals exposed to Se nanoparticles or to selenite. Based on effects on body weight and liver weight, selenium nanoparticles and ionic Se exerted similar toxicity. This suggests that a nanoparticle-specific toxicity of Se did not occur.",
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author = "Niels Hadrup and Katrin L{\"o}schner and {Mandrup Egebjerg}, Karen and Gitte Ravn-Haren and Frandsen, {Henrik Lauritz} and Larsen, {Erik Huusfeldt} and Lam, {Henrik R.} and Alicja Mortensen",
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language = "English",
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Subacute oral toxicity investigation of selenium nanoparticles and selenite in rats. / Hadrup, Niels; Löschner, Katrin; Mandrup Egebjerg, Karen; Ravn-Haren, Gitte; Frandsen, Henrik Lauritz; Larsen, Erik Huusfeldt; Lam, Henrik R.; Mortensen, Alicja.

In: Drug and Chemical Toxicology, Vol. 42, No. 1, 2019, p. 76-83.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Subacute oral toxicity investigation of selenium nanoparticles and selenite in rats

AU - Hadrup, Niels

AU - Löschner, Katrin

AU - Mandrup Egebjerg, Karen

AU - Ravn-Haren, Gitte

AU - Frandsen, Henrik Lauritz

AU - Larsen, Erik Huusfeldt

AU - Lam, Henrik R.

AU - Mortensen, Alicja

PY - 2019

Y1 - 2019

N2 - Selenium (Se) nanoparticles have been proposed as food supplements. However, the particle formulation may exert unexpected toxicity. The aim was therefore to compare toxicity of low doses of Se nanoparticles and the dissolved, ionized Se species selenite. Female rats were dosed orally for 28 d with either: 0.05, 0.5, or 4 mg Se/kg body weight (bw)/day as 20 nm Se nanoparticles or 0.05 or 0.5 mg Se/kg bw/day as sodium selenite. Male rats were dosed 4 mg Se/kg bw/day as Se nanoparticles. Body weight and clinical appearance were recorded throughout the experiment. At necropsy, blood samples were taken for hematological and clinical chemistry analyses; organ weights were recorded. At the high-dose of Se nanoparticles, overt toxicity occurred and the female animals had to be euthanized prematurely, whereas the male animals were reduced in dose. At all doses of Se nanoparticles and at 0.5 mg Se/kg bw/day as selenite, a lower body weight gain as compared to vehicle occurred. Relative liver weight was increased for both Se formulations at 0.5 mg Se/kg bw/day. Creatinine clearance and urinary pH were affected in some Se dosed groups. There were no effects among dosed groups on brain neurotransmitters or on hematological parameters compared with controls. There were no histological changes in the livers of animals exposed to Se nanoparticles or to selenite. Based on effects on body weight and liver weight, selenium nanoparticles and ionic Se exerted similar toxicity. This suggests that a nanoparticle-specific toxicity of Se did not occur.

AB - Selenium (Se) nanoparticles have been proposed as food supplements. However, the particle formulation may exert unexpected toxicity. The aim was therefore to compare toxicity of low doses of Se nanoparticles and the dissolved, ionized Se species selenite. Female rats were dosed orally for 28 d with either: 0.05, 0.5, or 4 mg Se/kg body weight (bw)/day as 20 nm Se nanoparticles or 0.05 or 0.5 mg Se/kg bw/day as sodium selenite. Male rats were dosed 4 mg Se/kg bw/day as Se nanoparticles. Body weight and clinical appearance were recorded throughout the experiment. At necropsy, blood samples were taken for hematological and clinical chemistry analyses; organ weights were recorded. At the high-dose of Se nanoparticles, overt toxicity occurred and the female animals had to be euthanized prematurely, whereas the male animals were reduced in dose. At all doses of Se nanoparticles and at 0.5 mg Se/kg bw/day as selenite, a lower body weight gain as compared to vehicle occurred. Relative liver weight was increased for both Se formulations at 0.5 mg Se/kg bw/day. Creatinine clearance and urinary pH were affected in some Se dosed groups. There were no effects among dosed groups on brain neurotransmitters or on hematological parameters compared with controls. There were no histological changes in the livers of animals exposed to Se nanoparticles or to selenite. Based on effects on body weight and liver weight, selenium nanoparticles and ionic Se exerted similar toxicity. This suggests that a nanoparticle-specific toxicity of Se did not occur.

KW - Selenium

KW - Nanoparticle

KW - Sodium selenite

KW - Toxicity

KW - Subacute

U2 - 10.1080/01480545.2018.1491589

DO - 10.1080/01480545.2018.1491589

M3 - Journal article

VL - 42

SP - 76

EP - 83

JO - Drug and Chemical Toxicology

JF - Drug and Chemical Toxicology

SN - 0148-0545

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ER -