Study on Cortisol Sensing Principle Based on Fluorophore and Aptamer Competitive Assay on Polymer Optical Fiber

Vivek Semwal*, Jonas Højgaard, Emil Møller, Ole Bang, Jakob Janting

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

37 Downloads (Pure)

Abstract

In this study, we present a polymer optical fiber fluorophore/aptamer competitive assaybased cortisol sensing principle. We developed a low-cost, two-fiber perpendicular design for fluorophore-based sensing with less input light interference and high output signal intensity. The design is suitable for narrow stokes shift fluorophores. We have demonstrated the cortisol sensing principle based on the competition between tagged and normal cortisol. To date, the sensing design has exhibited a slow response, and we identified possible modifications for improvement. Our estimation shows that with miniaturization and a modified sensor assay compartment design, a less than one-hour response time can be achieved. The reported sensing principle and low-cost new design will be helpful for the future development of fluorophore-based fiber optic aptasensors that can potentially be used in a wet environment for online sensing
Original languageEnglish
JournalPhotonics
Volume10
Issue number7
Number of pages10
ISSN2304-6732
DOIs
Publication statusPublished - 2023

Keywords

  • Fluorophores
  • Aptamer
  • Optical fiber
  • Competitive assay
  • Sensing

Fingerprint

Dive into the research topics of 'Study on Cortisol Sensing Principle Based on Fluorophore and Aptamer Competitive Assay on Polymer Optical Fiber'. Together they form a unique fingerprint.

Cite this