Study of the new electron transfer mediators in glucose oxidase catalysis

J. Kulys; T. Buch-Rasmussen; K. Bechgaard; V. Razamas; J. Kazlauskaite; J. Marcinkeviciene; J.B. Christensen; H.E. Hansen

doi:10.1016/0304-5102(94)00042-5

Study of the new electron transfer mediators in glucose oxidase catalysis

J. Kulys
, T. Buch-Rasmussen
, K. Bechgaard
, V. Razamas
, J. Kazlauskaite
, J. Marcinkeviciene
, J.B. Christensen
, H.E. Hansen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The steady-state oxidation of glucose oxidase from Aspergillus niger by phenothiazines, phenoxazines, Wurster's salts, dithia- and tetrathiaaromatic compounds, and nickelocene was investigated spectrophotometrically and electrochemically. At pH 7.0 the determined oxidation rate constants (TN/K_m) vary in the range 10³ to 10⁸ M⁻¹·s⁻¹. For phenothiazines, phenoxazine and Wurster's salts oxidation constants depend on the redox potential of the electron acceptors, and results were interpreted in the framework of the outer sphere electron transfer theory (Marcus and Sutin, Biochim. Biophys. Acta, 811 (1985) 265). The interpretation of the kinetic results concerning thiaaromatic compounds and metallocenes are complicated due to complex formation with the enzyme active center and aggregation of their oxidized form in buffer solution.

Original language	English
Journal	Journal of Molecular Catalysis
Volume	91
Issue number	3
Pages (from-to)	407-420
ISSN	0304-5102
DOIs	https://doi.org/10.1016/0304-5102(94)00042-5
Publication status	Published - 1994

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title = "Study of the new electron transfer mediators in glucose oxidase catalysis",

abstract = "The steady-state oxidation of glucose oxidase from Aspergillus niger by phenothiazines, phenoxazines, Wurster's salts, dithia- and tetrathiaaromatic compounds, and nickelocene was investigated spectrophotometrically and electrochemically. At pH 7.0 the determined oxidation rate constants (TN/Km) vary in the range 103 to 108 M−1·s−1. For phenothiazines, phenoxazine and Wurster's salts oxidation constants depend on the redox potential of the electron acceptors, and results were interpreted in the framework of the outer sphere electron transfer theory (Marcus and Sutin, Biochim. Biophys. Acta, 811 (1985) 265). The interpretation of the kinetic results concerning thiaaromatic compounds and metallocenes are complicated due to complex formation with the enzyme active center and aggregation of their oxidized form in buffer solution.",

keywords = "Materialer med s{\ae}rlige fysiske og kemiske egenskaber",

author = "J. Kulys and T. Buch-Rasmussen and K. Bechgaard and V. Razamas and J. Kazlauskaite and J. Marcinkeviciene and J.B. Christensen and H.E. Hansen",

year = "1994",

doi = "10.1016/0304-5102(94)00042-5",

language = "English",

volume = "91",

pages = "407--420",

journal = "Journal of Molecular Catalysis",

issn = "0304-5102",

publisher = "Elsevier",

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TY - JOUR

T1 - Study of the new electron transfer mediators in glucose oxidase catalysis

AU - Kulys, J.

AU - Buch-Rasmussen, T.

AU - Bechgaard, K.

AU - Razamas, V.

AU - Kazlauskaite, J.

AU - Marcinkeviciene, J.

AU - Christensen, J.B.

AU - Hansen, H.E.

PY - 1994

Y1 - 1994

N2 - The steady-state oxidation of glucose oxidase from Aspergillus niger by phenothiazines, phenoxazines, Wurster's salts, dithia- and tetrathiaaromatic compounds, and nickelocene was investigated spectrophotometrically and electrochemically. At pH 7.0 the determined oxidation rate constants (TN/Km) vary in the range 103 to 108 M−1·s−1. For phenothiazines, phenoxazine and Wurster's salts oxidation constants depend on the redox potential of the electron acceptors, and results were interpreted in the framework of the outer sphere electron transfer theory (Marcus and Sutin, Biochim. Biophys. Acta, 811 (1985) 265). The interpretation of the kinetic results concerning thiaaromatic compounds and metallocenes are complicated due to complex formation with the enzyme active center and aggregation of their oxidized form in buffer solution.

AB - The steady-state oxidation of glucose oxidase from Aspergillus niger by phenothiazines, phenoxazines, Wurster's salts, dithia- and tetrathiaaromatic compounds, and nickelocene was investigated spectrophotometrically and electrochemically. At pH 7.0 the determined oxidation rate constants (TN/Km) vary in the range 103 to 108 M−1·s−1. For phenothiazines, phenoxazine and Wurster's salts oxidation constants depend on the redox potential of the electron acceptors, and results were interpreted in the framework of the outer sphere electron transfer theory (Marcus and Sutin, Biochim. Biophys. Acta, 811 (1985) 265). The interpretation of the kinetic results concerning thiaaromatic compounds and metallocenes are complicated due to complex formation with the enzyme active center and aggregation of their oxidized form in buffer solution.

KW - Materialer med særlige fysiske og kemiske egenskaber

U2 - 10.1016/0304-5102(94)00042-5

DO - 10.1016/0304-5102(94)00042-5

M3 - Journal article

SN - 0304-5102

VL - 91

SP - 407

EP - 420

JO - Journal of Molecular Catalysis

JF - Journal of Molecular Catalysis

IS - 3

ER -

Study of the new electron transfer mediators in glucose oxidase catalysis

Abstract

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