Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

Julie Kirkegaard, Casper Hyttel Clausen, Romén Rodriguez-Trujíllo, Winnie Edith Svendsen

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    Abstract

    This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.
    Original languageEnglish
    JournalBiosensors
    Volume4
    Issue number3
    Pages (from-to)257-272
    Number of pages16
    ISSN2079-6374
    DOIs
    Publication statusPublished - 2014

    Bibliographical note

    Creative Commons Attribution-NonCommercial-ShareAlike 2.5 License

    Keywords

    • Electrical impedance spectroscopy
    • HeLa cells
    • Paclitaxel
    • Cell properties

    Cite this

    Kirkegaard, Julie ; Clausen, Casper Hyttel ; Rodriguez-Trujíllo, Romén ; Svendsen, Winnie Edith. / Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry. In: Biosensors. 2014 ; Vol. 4, No. 3. pp. 257-272.
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    abstract = "This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.",
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    Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry. / Kirkegaard, Julie; Clausen, Casper Hyttel; Rodriguez-Trujíllo, Romén; Svendsen, Winnie Edith.

    In: Biosensors, Vol. 4, No. 3, 2014, p. 257-272.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    AU - Kirkegaard, Julie

    AU - Clausen, Casper Hyttel

    AU - Rodriguez-Trujíllo, Romén

    AU - Svendsen, Winnie Edith

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    AB - This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.

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