Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

Julie Kirkegaard; Casper Hyttel Clausen; Romén Rodriguez-Trujíllo; Winnie Edith Svendsen

doi:10.3390/bios4030257

Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

Julie Kirkegaard, Casper Hyttel Clausen, Romén Rodriguez-Trujíllo, Winnie Edith Svendsen

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Abstract

This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.

Original language	English
Journal	Biosensors
Volume	4
Issue number	3
Pages (from-to)	257-272
Number of pages	16
ISSN	2079-6374
DOIs	https://doi.org/10.3390/bios4030257
Publication status	Published - 2014

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Creative Commons Attribution-NonCommercial-ShareAlike 2.5 License

Keywords

Electrical impedance spectroscopy
HeLa cells
Paclitaxel
Cell properties

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10.3390/bios4030257

Biosensors 04 00257 1Final published version, 1.15 MB

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title = "Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry",

abstract = "This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.",

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N2 - This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.

AB - This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.

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Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

Abstract

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