Barley limit dextrinase (HvLD) is a debranching enzyme from glycoside hydrolase family 13 subfamily 13 (GH13_13) that hydrolyses α-1,6-glucosidic linkages in limit dextrins derived from amylopectin. The structure of HvLD was solved and refined to 1.9 Å resolution. The structure has a glycerol molecule in the active site and is virtually identical to the structures of HvLD in complex with the competitive inhibitors α-cyclodextrin and β-cyclodextrin solved to 2.5 and 2.1 Å resolution, respectively. However, three loops in the N-terminal domain that are shown here to resemble carbohydrate-binding module family 21 were traceable and were included in the present HvLD structure but were too flexible to be traced and included in the structures of the two HvLD-inhibitor complexes.
|Journal||Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online|
|Issue number||Pt 9|
|Publication status||Published - 2012|
Møller, M. S., Abou Hachem, M., Svensson, B., & Henriksen, A. (2012). Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online, 68(Pt 9), 1008-1012. https://doi.org/10.1107/S1744309112031004