Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164

E. Vinogradov, Bent O. Petersen, I. Sadovskaya, S. Jabbouri, Jens Øllgaard Duus, IM Helander

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Ara4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R-1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100(T), showed that it contains a very similar core but with one different glycosidic linkage.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume270
Issue number14
Pages (from-to)3036-3046
Number of pages11
ISSN0014-2956
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • acetic acid
  • bacterium lipopolysaccharide
  • carbohydrate
  • glucose derivative
  • lipid A
  • monosaccharide
  • oligosaccharide
  • polysaccharide
  • article
  • bacterial strain
  • carbohydrate analysis
  • deacylation
  • Gram negative bacterium
  • hydrolysis
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • Pectinatus frisingensis
  • priority journal
  • structure analysis
  • Acetic Acid
  • Acetylation
  • Bacteria, Anaerobic
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Electrophoresis, Polyacrylamide Gel
  • Glycosides
  • Hydrolysis
  • Lipopolysaccharides
  • Methylglycosides
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Bacteria (microorganisms)
  • Negibacteria
  • Core
  • Lipid A
  • Lipopolysaccharide
  • 642-33-1 3-O-methyl-6-deoxy-D-talose
  • Q40Q9N063P Acetic Acid
  • core
  • lipopolysaccharide
  • BIOCHEMISTRY
  • GENUS PECTINATUS
  • LIPID-A
  • ACID
  • POLYSACCHARIDE
  • KDO
  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Pectinatus cerevisiiphilus species strain-E-79103 Pectinatus frisingensis species strain-VTT E-79100, strain-VTT E-82164
  • Pectinatus frisingensis lipopolysaccharide exceptionally large nonrepetitive carbohydrate backbone structure
  • 10060, Biochemistry studies - General
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • gel chromatography chromatographic techniques, laboratory techniques
  • high-performance ion exchange chromatography chromatographic techniques, laboratory techniques
  • mass spectrometry laboratory techniques, spectrum analysis techniques
  • NMR spectroscopy laboratory techniques, spectrum analysis techniques
  • polyacrylamide gel electrophoresis electrophoretic techniques, laboratory techniques
  • Bacteriology
  • Biochemistry and Molecular Biophysics
  • OLIGOSACCHARIDES

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