Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164

E. Vinogradov; Bent O. Petersen; I. Sadovskaya; S. Jabbouri; Jens Øllgaard Duus; IM Helander

doi:10.1046/j.1432-1033.2003.03682.x

Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164

E. Vinogradov, Bent O. Petersen, I. Sadovskaya, S. Jabbouri, Jens Øllgaard Duus, IM Helander

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Ara4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R-1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100(T), showed that it contains a very similar core but with one different glycosidic linkage.

Original language	English
Journal	European Journal of Biochemistry
Volume	270
Issue number	14
Pages (from-to)	3036-3046
Number of pages	11
ISSN	0014-2956
DOIs	https://doi.org/10.1046/j.1432-1033.2003.03682.x
Publication status	Published - 2003
Externally published	Yes

Keywords

acetic acid
bacterium lipopolysaccharide
carbohydrate
glucose derivative
lipid A
monosaccharide
oligosaccharide
polysaccharide
article
bacterial strain
carbohydrate analysis
deacylation
Gram negative bacterium
hydrolysis
nonhuman
nuclear magnetic resonance spectroscopy
Pectinatus frisingensis
priority journal
structure analysis
Acetic Acid
Acetylation
Bacteria, Anaerobic
Carbohydrate Conformation
Carbohydrate Sequence
Electrophoresis, Polyacrylamide Gel
Glycosides
Hydrolysis
Lipopolysaccharides
Methylglycosides
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Bacteria (microorganisms)
Negibacteria
Core
Lipid A
Lipopolysaccharide
642-33-1 3-O-methyl-6-deoxy-D-talose
Q40Q9N063P Acetic Acid
core
lipopolysaccharide
BIOCHEMISTRY
GENUS PECTINATUS
LIPID-A
ACID
POLYSACCHARIDE
KDO
Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Pectinatus cerevisiiphilus species strain-E-79103 Pectinatus frisingensis species strain-VTT E-79100, strain-VTT E-82164
Pectinatus frisingensis lipopolysaccharide exceptionally large nonrepetitive carbohydrate backbone structure
10060, Biochemistry studies - General
30000, Bacteriology, general and systematic
31000, Physiology and biochemistry of bacteria
gel chromatography chromatographic techniques, laboratory techniques
high-performance ion exchange chromatography chromatographic techniques, laboratory techniques
mass spectrometry laboratory techniques, spectrum analysis techniques
NMR spectroscopy laboratory techniques, spectrum analysis techniques
polyacrylamide gel electrophoresis electrophoretic techniques, laboratory techniques
Bacteriology
Biochemistry and Molecular Biophysics
OLIGOSACCHARIDES

Access to Document

10.1046/j.1432-1033.2003.03682.x

OpenUrl availability

Full text

Cite this

@article{2dea891ff9594fe1b608e7c0db83ced9,

title = "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164",

abstract = "The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Ara4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R-1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100(T), showed that it contains a very similar core but with one different glycosidic linkage.",

keywords = "acetic acid, bacterium lipopolysaccharide, carbohydrate, glucose derivative, lipid A, monosaccharide, oligosaccharide, polysaccharide, article, bacterial strain, carbohydrate analysis, deacylation, Gram negative bacterium, hydrolysis, nonhuman, nuclear magnetic resonance spectroscopy, Pectinatus frisingensis, priority journal, structure analysis, Acetic Acid, Acetylation, Bacteria, Anaerobic, Carbohydrate Conformation, Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Glycosides, Hydrolysis, Lipopolysaccharides, Methylglycosides, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Bacteria (microorganisms), Negibacteria, Core, Lipid A, Lipopolysaccharide, 642-33-1 3-O-methyl-6-deoxy-D-talose, Q40Q9N063P Acetic Acid, core, lipopolysaccharide, BIOCHEMISTRY, GENUS PECTINATUS, LIPID-A, ACID, POLYSACCHARIDE, KDO, Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Pectinatus cerevisiiphilus species strain-E-79103 Pectinatus frisingensis species strain-VTT E-79100, strain-VTT E-82164, Pectinatus frisingensis lipopolysaccharide exceptionally large nonrepetitive carbohydrate backbone structure, 10060, Biochemistry studies - General, 30000, Bacteriology, general and systematic, 31000, Physiology and biochemistry of bacteria, gel chromatography chromatographic techniques, laboratory techniques, high-performance ion exchange chromatography chromatographic techniques, laboratory techniques, mass spectrometry laboratory techniques, spectrum analysis techniques, NMR spectroscopy laboratory techniques, spectrum analysis techniques, polyacrylamide gel electrophoresis electrophoretic techniques, laboratory techniques, Bacteriology, Biochemistry and Molecular Biophysics, OLIGOSACCHARIDES",

author = "E. Vinogradov and Petersen, {Bent O.} and I. Sadovskaya and S. Jabbouri and Duus, {Jens {\O}llgaard} and IM Helander",

year = "2003",

doi = "10.1046/j.1432-1033.2003.03682.x",

language = "English",

volume = "270",

pages = "3036--3046",

journal = "European Journal of Biochemistry",

issn = "0014-2956",

number = "14",

}

TY - JOUR

T1 - Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164

AU - Vinogradov, E.

AU - Petersen, Bent O.

AU - Sadovskaya, I.

AU - Jabbouri, S.

AU - Duus, Jens Øllgaard

AU - Helander, IM

PY - 2003

Y1 - 2003

N2 - The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Ara4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R-1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100(T), showed that it contains a very similar core but with one different glycosidic linkage.

AB - The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Ara4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R-1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100(T), showed that it contains a very similar core but with one different glycosidic linkage.

KW - acetic acid

KW - bacterium lipopolysaccharide

KW - carbohydrate

KW - glucose derivative

KW - lipid A

KW - monosaccharide

KW - oligosaccharide

KW - polysaccharide

KW - article

KW - bacterial strain

KW - carbohydrate analysis

KW - deacylation

KW - Gram negative bacterium

KW - hydrolysis

KW - nonhuman

KW - nuclear magnetic resonance spectroscopy

KW - Pectinatus frisingensis

KW - priority journal

KW - structure analysis

KW - Acetic Acid

KW - Acetylation

KW - Bacteria, Anaerobic

KW - Carbohydrate Conformation

KW - Carbohydrate Sequence

KW - Electrophoresis, Polyacrylamide Gel

KW - Glycosides

KW - Hydrolysis

KW - Lipopolysaccharides

KW - Methylglycosides

KW - Molecular Sequence Data

KW - Nuclear Magnetic Resonance, Biomolecular

KW - Bacteria (microorganisms)

KW - Negibacteria

KW - Core

KW - Lipid A

KW - Lipopolysaccharide

KW - 642-33-1 3-O-methyl-6-deoxy-D-talose

KW - Q40Q9N063P Acetic Acid

KW - core

KW - lipopolysaccharide

KW - BIOCHEMISTRY

KW - GENUS PECTINATUS

KW - LIPID-A

KW - ACID

KW - POLYSACCHARIDE

KW - KDO

KW - Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Pectinatus cerevisiiphilus species strain-E-79103 Pectinatus frisingensis species strain-VTT E-79100, strain-VTT E-82164

KW - Pectinatus frisingensis lipopolysaccharide exceptionally large nonrepetitive carbohydrate backbone structure

KW - 10060, Biochemistry studies - General

KW - 30000, Bacteriology, general and systematic

KW - 31000, Physiology and biochemistry of bacteria

KW - gel chromatography chromatographic techniques, laboratory techniques

KW - high-performance ion exchange chromatography chromatographic techniques, laboratory techniques

KW - mass spectrometry laboratory techniques, spectrum analysis techniques

KW - NMR spectroscopy laboratory techniques, spectrum analysis techniques

KW - polyacrylamide gel electrophoresis electrophoretic techniques, laboratory techniques

KW - Bacteriology

KW - Biochemistry and Molecular Biophysics

KW - OLIGOSACCHARIDES

U2 - 10.1046/j.1432-1033.2003.03682.x

DO - 10.1046/j.1432-1033.2003.03682.x

M3 - Journal article

SN - 0014-2956

VL - 270

SP - 3036

EP - 3046

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 14

ER -

Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164

Abstract

Keywords

Access to Document

OpenUrl availability

Fingerprint

Cite this