Structure of plastocyanin from the cyanobacterium Anabaena variabilis

Lars Schmidt, Hans Erik Mølager Christensen, Pernille Harris

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in E. coli and purified. Plate-like crystals were obtained by crystallisation in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P212121 with cell dimensions a = 67.85 Å, b = 45.81 Å and c = 63.41 Å. The structure of the oxidised protein was solved to a resolution of 1.6 Å using plastocyanin from Phormidium laminosum as search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and two distinct conformations of His61.
Original languageEnglish
JournalActa Crystallographica. Section D: Biological Crystallography
Volume62
Issue number9
Pages (from-to)1022-1029
ISSN0907-4449
DOIs
Publication statusPublished - 2006

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