Structural Organization of pRAM4, a Cryptic Plasmid from Prevotella ruminicola

Koretsugu Ogata; Rustam Aminov; Takafumi Nagamine; Yoshimi Benno; Tsutomu Sekizaki; Makoto Mitsumori; Hajime Minato; Hisao Itabashi

doi:10.1006/plas.1996.0011

Structural Organization of pRAM4, a Cryptic Plasmid from Prevotella ruminicola

Koretsugu Ogata, Rustam Aminov, Takafumi Nagamine, Yoshimi Benno, Tsutomu Sekizaki, Makoto Mitsumori, Hajime Minato, Hisao Itabashi

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Abstract

A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reaading frames, encoding potential polypeptides of Mr 32,322 (ORF1) and 32,122 (ORF2) with limited sequency similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequene. The region upstream from ORF1 had an AT-rich (5%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.

Original language	English
Journal	Plasmid
Volume	35
Issue number	2
Pages (from-to)	91-97
ISSN	0147-619X
DOIs	https://doi.org/10.1006/plas.1996.0011
Publication status	Published - 1996
Externally published	Yes

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@article{a291117e0bd641df876f937817c1f988,

title = "Structural Organization of pRAM4, a Cryptic Plasmid from Prevotella ruminicola",

abstract = "A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reaading frames, encoding potential polypeptides of Mr 32,322 (ORF1) and 32,122 (ORF2) with limited sequency similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequene. The region upstream from ORF1 had an AT-rich (5%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.",

author = "Koretsugu Ogata and Rustam Aminov and Takafumi Nagamine and Yoshimi Benno and Tsutomu Sekizaki and Makoto Mitsumori and Hajime Minato and Hisao Itabashi",

year = "1996",

doi = "10.1006/plas.1996.0011",

language = "English",

volume = "35",

pages = "91--97",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press",

number = "2",

}

TY - JOUR

T1 - Structural Organization of pRAM4, a Cryptic Plasmid from Prevotella ruminicola

AU - Ogata, Koretsugu

AU - Aminov, Rustam

AU - Nagamine, Takafumi

AU - Benno, Yoshimi

AU - Sekizaki, Tsutomu

AU - Mitsumori, Makoto

AU - Minato, Hajime

AU - Itabashi, Hisao

PY - 1996

Y1 - 1996

N2 - A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reaading frames, encoding potential polypeptides of Mr 32,322 (ORF1) and 32,122 (ORF2) with limited sequency similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequene. The region upstream from ORF1 had an AT-rich (5%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.

AB - A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reaading frames, encoding potential polypeptides of Mr 32,322 (ORF1) and 32,122 (ORF2) with limited sequency similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequene. The region upstream from ORF1 had an AT-rich (5%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.

U2 - 10.1006/plas.1996.0011

DO - 10.1006/plas.1996.0011

M3 - Journal article

C2 - 8700970

VL - 35

SP - 91

EP - 97

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 2

ER -