TY - JOUR
T1 - Structural Organization of pRAM4, a Cryptic Plasmid from Prevotella ruminicola
AU - Ogata, Koretsugu
AU - Aminov, Rustam
AU - Nagamine, Takafumi
AU - Benno, Yoshimi
AU - Sekizaki, Tsutomu
AU - Mitsumori, Makoto
AU - Minato, Hajime
AU - Itabashi, Hisao
PY - 1996
Y1 - 1996
N2 - A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reaading frames, encoding potential polypeptides of Mr 32,322 (ORF1) and 32,122 (ORF2) with limited sequency similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequene. The region upstream from ORF1 had an AT-rich (5%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.
AB - A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reaading frames, encoding potential polypeptides of Mr 32,322 (ORF1) and 32,122 (ORF2) with limited sequency similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequene. The region upstream from ORF1 had an AT-rich (5%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.
U2 - 10.1006/plas.1996.0011
DO - 10.1006/plas.1996.0011
M3 - Journal article
C2 - 8700970
VL - 35
SP - 91
EP - 97
JO - Plasmid
JF - Plasmid
SN - 0147-619X
IS - 2
ER -