Structural Insights into Substrate Specificity and the anti beta-Elimination Mechanism of Pectate Lyase

A. Seyedarabi, T.T. To, S. Ali, S. Hussain, M. Fries, Robert Madsen, Mads Hartvig Clausen, S. Teixteira, K. Brocklehurst, R.W. Pickersgill

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Pectate lyases harness anti beta-elimination chemistry to cleave the alpha-1,4 linkage in the homogalacturonan region of plant cell Wall pectin. We have studied the binding of five pectic oligosaccharides to Bacillus subtilis pectate Iyase in crystals of the inactive enzyme in which the catalytic base is substituted with alanine (R279A). We discover that the three central subsites (- 1, + 1, and +2) have a profound preference for galacturonate but that the distal subsites call accommodate methylated galactUronate. h Is reasonable to assume therefore that pectate Iyase call cleave pectin with three consecutive galacturonate residues. The enzyme in the absence Of Substrate binds I single calcium Ion, and we show that two additional calcium ions bind between enzyme Mid Substrate carboxylates Occupying the +1 subsite in the Michaelis complex. The Substrate binds less Intimately to the enzyme in a complex made with a catalytic base in place but In the absence of the calcium ions and an adjacent lysine, In this complex, the catalytic base is correctly positioned to abstract the C5 proton, but there are no calcium ions during the carboxylate at the +I subsite. It is clear, therefore, that the catalytic calcium ions and adjacent lysine promote catalysts by acidifying (lie a-proton, Facilitating its abstraction by the base. There is also clear evidence that binding distorts the relaxed 21 or 31 helical conformation of the of oligosaccharides in the region of the scissile bond.
Original languageEnglish
JournalBiochemistry
Volume49
Issue number3
Pages (from-to)539-546
ISSN0006-2960
DOIs
Publication statusPublished - 2010

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