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Structural determination of Streptococcus pneumoniae repeat units in serotype 41A and 41F capsular polysaccharides to probe gene functions in the corresponding capsular biosynthetic loci. / Petersen, Bent O.; Skovsted, Ian C.; Paulsen, Berit Smestad; Redondo, Antonio R.; Meier, Sebastian.

In: Carbohydrate Research, Vol. 400, 2014, p. 26–32.

Research output: Contribution to journalJournal article – Annual report year: 2014Researchpeer-review

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@article{b44bb20f2f5147788bf9a63bf4dbbdef,
title = "Structural determination of Streptococcus pneumoniae repeat units in serotype 41A and 41F capsular polysaccharides to probe gene functions in the corresponding capsular biosynthetic loci",
abstract = "We report the repeating unit structures ofthe native capsular polysaccharidesof S. pneumoniaeserotypes 41A and 41F. Structuraldeterminationsyieldedsix carbohydrate units in the doubly branched repeating unit to givethe following structure for serotype 41A:The structure determinations were motivated (1) by anambitionto help close the remaining gaps in S. pneumoniaecapsular polysaccharide structures, and (2)by the attempt to derive functional annotationsof carbohydrate active enzymes in the biosynthesis of bacterial polysaccharides from the determined structures. Anactivitypresent in 41F but not 41Ais identified as an acetyltransferaseacting on therhamnopyranosoyl sidechain E. The genes encoding theformation of thesix glycosidic bondsin serogroup 41 weredetermined from thecapsular polysaccharide structuresofserotype 41A,41F and genetically related serotypes,in conjunction with corresponding genomic information and computational homology searches. In combination with complementary information, NMR spectroscopy considerably simplifiesthe functional annotation of carbohydrate active enzymes in the biosynthesis of bacterial polysaccharides",
keywords = "Biosynthetic loci, Capsular polysaccharide, NMR, Serotype 41A, Streptoccocus pneumonia e",
author = "Petersen, {Bent O.} and Skovsted, {Ian C.} and Paulsen, {Berit Smestad} and Redondo, {Antonio R.} and Sebastian Meier",
year = "2014",
doi = "10.1016/j.carres.2014.08.017",
language = "English",
volume = "400",
pages = "26–32",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Structural determination of Streptococcus pneumoniae repeat units in serotype 41A and 41F capsular polysaccharides to probe gene functions in the corresponding capsular biosynthetic loci

AU - Petersen, Bent O.

AU - Skovsted, Ian C.

AU - Paulsen, Berit Smestad

AU - Redondo, Antonio R.

AU - Meier, Sebastian

PY - 2014

Y1 - 2014

N2 - We report the repeating unit structures ofthe native capsular polysaccharidesof S. pneumoniaeserotypes 41A and 41F. Structuraldeterminationsyieldedsix carbohydrate units in the doubly branched repeating unit to givethe following structure for serotype 41A:The structure determinations were motivated (1) by anambitionto help close the remaining gaps in S. pneumoniaecapsular polysaccharide structures, and (2)by the attempt to derive functional annotationsof carbohydrate active enzymes in the biosynthesis of bacterial polysaccharides from the determined structures. Anactivitypresent in 41F but not 41Ais identified as an acetyltransferaseacting on therhamnopyranosoyl sidechain E. The genes encoding theformation of thesix glycosidic bondsin serogroup 41 weredetermined from thecapsular polysaccharide structuresofserotype 41A,41F and genetically related serotypes,in conjunction with corresponding genomic information and computational homology searches. In combination with complementary information, NMR spectroscopy considerably simplifiesthe functional annotation of carbohydrate active enzymes in the biosynthesis of bacterial polysaccharides

AB - We report the repeating unit structures ofthe native capsular polysaccharidesof S. pneumoniaeserotypes 41A and 41F. Structuraldeterminationsyieldedsix carbohydrate units in the doubly branched repeating unit to givethe following structure for serotype 41A:The structure determinations were motivated (1) by anambitionto help close the remaining gaps in S. pneumoniaecapsular polysaccharide structures, and (2)by the attempt to derive functional annotationsof carbohydrate active enzymes in the biosynthesis of bacterial polysaccharides from the determined structures. Anactivitypresent in 41F but not 41Ais identified as an acetyltransferaseacting on therhamnopyranosoyl sidechain E. The genes encoding theformation of thesix glycosidic bondsin serogroup 41 weredetermined from thecapsular polysaccharide structuresofserotype 41A,41F and genetically related serotypes,in conjunction with corresponding genomic information and computational homology searches. In combination with complementary information, NMR spectroscopy considerably simplifiesthe functional annotation of carbohydrate active enzymes in the biosynthesis of bacterial polysaccharides

KW - Biosynthetic loci

KW - Capsular polysaccharide

KW - NMR

KW - Serotype 41A

KW - Streptoccocus pneumonia e

U2 - 10.1016/j.carres.2014.08.017

DO - 10.1016/j.carres.2014.08.017

M3 - Journal article

VL - 400

SP - 26

EP - 32

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

ER -