Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

Bent O. Petersen; Margit Sára; Christoph Mader; Harald F. Mayer; Uwe B. Sleytr; Martin Pabst; Michael Puchberger; Eberhard Krause; Andreas Hofinger; Jens Øllgaard Duus; Paul Kosma

doi:10.1016/j.carres.2008.03.029

Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

Bent O. Petersen
, Margit Sára
, Christoph Mader
, Harald F. Mayer
, Uwe B. Sleytr
, Martin Pabst
, Michael Puchberger
, Eberhard Krause
, Andreas Hofinger
, Jens Øllgaard Duus
, Paul Kosma

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide—liberated by HF-treatment from the cell wall—was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:

Original language	English
Journal	Carbohydrate Research
Volume	343
Issue number	8
Pages (from-to)	1346-1358
ISSN	0008-6215
DOIs	https://doi.org/10.1016/j.carres.2008.03.029
Publication status	Published - 2008
Externally published	Yes

Keywords

Geobacillus
Secondary cell wall polymer
NMR spectroscopy
Mass spectrometry
Polysaccharide structure

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10.1016/j.carres.2008.03.029

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@article{994ec7ca5660456f847a9f6aa85e355d,

title = "Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2",

abstract = "The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide—liberated by HF-treatment from the cell wall—was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:",

keywords = "Geobacillus, Secondary cell wall polymer, NMR spectroscopy, Mass spectrometry, Polysaccharide structure",

author = "Petersen, \{Bent O.\} and Margit S{\'a}ra and Christoph Mader and Mayer, \{Harald F.\} and Sleytr, \{Uwe B.\} and Martin Pabst and Michael Puchberger and Eberhard Krause and Andreas Hofinger and Duus, \{Jens {\O}llgaard\} and Paul Kosma",

year = "2008",

doi = "10.1016/j.carres.2008.03.029",

language = "English",

volume = "343",

pages = "1346--1358",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier",

number = "8",

}

TY - JOUR

T1 - Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

AU - Petersen, Bent O.

AU - Sára, Margit

AU - Mader, Christoph

AU - Mayer, Harald F.

AU - Sleytr, Uwe B.

AU - Pabst, Martin

AU - Puchberger, Michael

AU - Krause, Eberhard

AU - Hofinger, Andreas

AU - Duus, Jens Øllgaard

AU - Kosma, Paul

PY - 2008

Y1 - 2008

N2 - The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide—liberated by HF-treatment from the cell wall—was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:

AB - The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide—liberated by HF-treatment from the cell wall—was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:

KW - Geobacillus

KW - Secondary cell wall polymer

KW - NMR spectroscopy

KW - Mass spectrometry

KW - Polysaccharide structure

U2 - 10.1016/j.carres.2008.03.029

DO - 10.1016/j.carres.2008.03.029

M3 - Journal article

C2 - 18420185

SN - 0008-6215

VL - 343

SP - 1346

EP - 1358

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 8

ER -

Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

Abstract

Keywords

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