Structural Characterization of Peptide Antibodies

Anna Chailyan, Paolo Marcatili

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    The role of proteins as very effective immunogens for the generation of antibodies is indisputable. Nevertheless, cases in which protein usage for antibody production is not feasible or convenient compelled the creation of a powerful alternative consisting of synthetic peptides. Synthetic peptides can be modified to obtain desired properties or conformation, tagged for purification, isotopically labeled for protein quantitation or conjugated to immunogens for antibody production. The antibodies that bind to these peptides represent an invaluable tool for biological research and discovery. To better understand the underlying mechanisms of antibody-antigen interaction here we present a pipeline developed by us to structurally classify immunoglobulin antigen binding sites and to infer key sequence residues and other variables that have a prominent role in each structural class.
    Original languageEnglish
    Title of host publicationPeptide Antibodies
    Number of pages10
    Volume1348
    PublisherSpringer
    Publication date2015
    Pages205-214
    Chapter18
    ISBN (Print)978-1-4939-2998-6
    ISBN (Electronic)978-1-4939-2999-3
    DOIs
    Publication statusPublished - 2015
    SeriesMethods in Molecular Biology
    ISSN1064-3745

    Keywords

    • Clustering
    • Linear epitope
    • Peptide antibody
    • Structure

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