Structural characterization of bioactive heteropolysaccharides from the medicinal fungus Inonotus obliquus (Chaga)

Christian Winther Wold*, Christian Kjeldsen, Alexandre Corthay, Frode Rise, Bjørn E. Christensen, Jens Øllgaard Duus, Kari Tvete Inngjerdingen

*Corresponding author for this work

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Abstract

The aim of this paper was to perform a comprehensive characterization of polysaccharides isolated from the interior (IOI) and exterior (IOE) parts of the fungus Inonotus obliquus. Pre-extraction with DCM and MeOH, followed by water and alkali extraction and ethanol precipitation gave two water extracts and two alkali extracts. Neutral and acidic polysaccharide fractions were obtained after anion-exchange chromatography of the water extracts. The neutral polysaccharides (60–73 kDa) were heterogeneous and branched and consisted of a (1 → 3)-linked β-Glc backbone with (1 → 6)-linked kinks in the chain at approximately every fifth residue, with branches of (1 → 6)-linked β-Glc in addition to substantial amounts of (1 → 6)-linked α-Gal with 3-O-methylation at about every third Gal residue. The acidic polysaccharide fractions (10–31 kDa) showed similar structural motifs as the neutral fractions differing mainly by the presence of (1 → 4)-linked α-GalA and α-GlcA. β-Xyl, α-Man and α-Rha were also present in varying amounts in all fractions. No major structural differences between the IOI and IOE fractions were observed. An alkaline polysaccharide fraction (>450 kDa) was obtained from the IOI alkali extract, and consisted mainly of (1 → 3)- and (1 → 6)-linked β-Glc and (1 → 4)-linked β-Xyl. Several of the fractions showed in vitro immunomodulatory effect by increasing NO production in the murine macrophage and dendritic cell lines J774.A1 and D2SC/1. Most fractions managed to increase NO production only at the highest concentration tested (100 μg/ml), while the neutral fraction IOE-WN activated potent NO production at 10 μg/ml and was considered the most promising immunomodulating fraction in this study.
Original languageEnglish
JournalCarbohydrate Polymers
Volume185
Pages (from-to)27-40
ISSN0144-8617
DOIs
Publication statusPublished - 2018

Keywords

  • Chaga
  • Fungi
  • Polysaccharides
  • Structural characterization
  • Galactoglucan
  • NMR

Cite this

Wold, Christian Winther ; Kjeldsen, Christian ; Corthay, Alexandre ; Rise, Frode ; Christensen, Bjørn E. ; Duus, Jens Øllgaard ; Inngjerdingen, Kari Tvete. / Structural characterization of bioactive heteropolysaccharides from the medicinal fungus Inonotus obliquus (Chaga). In: Carbohydrate Polymers. 2018 ; Vol. 185. pp. 27-40.
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abstract = "The aim of this paper was to perform a comprehensive characterization of polysaccharides isolated from the interior (IOI) and exterior (IOE) parts of the fungus Inonotus obliquus. Pre-extraction with DCM and MeOH, followed by water and alkali extraction and ethanol precipitation gave two water extracts and two alkali extracts. Neutral and acidic polysaccharide fractions were obtained after anion-exchange chromatography of the water extracts. The neutral polysaccharides (60–73 kDa) were heterogeneous and branched and consisted of a (1 → 3)-linked β-Glc backbone with (1 → 6)-linked kinks in the chain at approximately every fifth residue, with branches of (1 → 6)-linked β-Glc in addition to substantial amounts of (1 → 6)-linked α-Gal with 3-O-methylation at about every third Gal residue. The acidic polysaccharide fractions (10–31 kDa) showed similar structural motifs as the neutral fractions differing mainly by the presence of (1 → 4)-linked α-GalA and α-GlcA. β-Xyl, α-Man and α-Rha were also present in varying amounts in all fractions. No major structural differences between the IOI and IOE fractions were observed. An alkaline polysaccharide fraction (>450 kDa) was obtained from the IOI alkali extract, and consisted mainly of (1 → 3)- and (1 → 6)-linked β-Glc and (1 → 4)-linked β-Xyl. Several of the fractions showed in vitro immunomodulatory effect by increasing NO production in the murine macrophage and dendritic cell lines J774.A1 and D2SC/1. Most fractions managed to increase NO production only at the highest concentration tested (100 μg/ml), while the neutral fraction IOE-WN activated potent NO production at 10 μg/ml and was considered the most promising immunomodulating fraction in this study.",
keywords = "Chaga, Fungi, Polysaccharides, Structural characterization, Galactoglucan, NMR",
author = "Wold, {Christian Winther} and Christian Kjeldsen and Alexandre Corthay and Frode Rise and Christensen, {Bj{\o}rn E.} and Duus, {Jens {\O}llgaard} and Inngjerdingen, {Kari Tvete}",
year = "2018",
doi = "10.1016/j.carbpol.2017.12.041",
language = "English",
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Structural characterization of bioactive heteropolysaccharides from the medicinal fungus Inonotus obliquus (Chaga). / Wold, Christian Winther; Kjeldsen, Christian; Corthay, Alexandre; Rise, Frode; Christensen, Bjørn E.; Duus, Jens Øllgaard; Inngjerdingen, Kari Tvete.

In: Carbohydrate Polymers, Vol. 185, 2018, p. 27-40.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Structural characterization of bioactive heteropolysaccharides from the medicinal fungus Inonotus obliquus (Chaga)

AU - Wold, Christian Winther

AU - Kjeldsen, Christian

AU - Corthay, Alexandre

AU - Rise, Frode

AU - Christensen, Bjørn E.

AU - Duus, Jens Øllgaard

AU - Inngjerdingen, Kari Tvete

PY - 2018

Y1 - 2018

N2 - The aim of this paper was to perform a comprehensive characterization of polysaccharides isolated from the interior (IOI) and exterior (IOE) parts of the fungus Inonotus obliquus. Pre-extraction with DCM and MeOH, followed by water and alkali extraction and ethanol precipitation gave two water extracts and two alkali extracts. Neutral and acidic polysaccharide fractions were obtained after anion-exchange chromatography of the water extracts. The neutral polysaccharides (60–73 kDa) were heterogeneous and branched and consisted of a (1 → 3)-linked β-Glc backbone with (1 → 6)-linked kinks in the chain at approximately every fifth residue, with branches of (1 → 6)-linked β-Glc in addition to substantial amounts of (1 → 6)-linked α-Gal with 3-O-methylation at about every third Gal residue. The acidic polysaccharide fractions (10–31 kDa) showed similar structural motifs as the neutral fractions differing mainly by the presence of (1 → 4)-linked α-GalA and α-GlcA. β-Xyl, α-Man and α-Rha were also present in varying amounts in all fractions. No major structural differences between the IOI and IOE fractions were observed. An alkaline polysaccharide fraction (>450 kDa) was obtained from the IOI alkali extract, and consisted mainly of (1 → 3)- and (1 → 6)-linked β-Glc and (1 → 4)-linked β-Xyl. Several of the fractions showed in vitro immunomodulatory effect by increasing NO production in the murine macrophage and dendritic cell lines J774.A1 and D2SC/1. Most fractions managed to increase NO production only at the highest concentration tested (100 μg/ml), while the neutral fraction IOE-WN activated potent NO production at 10 μg/ml and was considered the most promising immunomodulating fraction in this study.

AB - The aim of this paper was to perform a comprehensive characterization of polysaccharides isolated from the interior (IOI) and exterior (IOE) parts of the fungus Inonotus obliquus. Pre-extraction with DCM and MeOH, followed by water and alkali extraction and ethanol precipitation gave two water extracts and two alkali extracts. Neutral and acidic polysaccharide fractions were obtained after anion-exchange chromatography of the water extracts. The neutral polysaccharides (60–73 kDa) were heterogeneous and branched and consisted of a (1 → 3)-linked β-Glc backbone with (1 → 6)-linked kinks in the chain at approximately every fifth residue, with branches of (1 → 6)-linked β-Glc in addition to substantial amounts of (1 → 6)-linked α-Gal with 3-O-methylation at about every third Gal residue. The acidic polysaccharide fractions (10–31 kDa) showed similar structural motifs as the neutral fractions differing mainly by the presence of (1 → 4)-linked α-GalA and α-GlcA. β-Xyl, α-Man and α-Rha were also present in varying amounts in all fractions. No major structural differences between the IOI and IOE fractions were observed. An alkaline polysaccharide fraction (>450 kDa) was obtained from the IOI alkali extract, and consisted mainly of (1 → 3)- and (1 → 6)-linked β-Glc and (1 → 4)-linked β-Xyl. Several of the fractions showed in vitro immunomodulatory effect by increasing NO production in the murine macrophage and dendritic cell lines J774.A1 and D2SC/1. Most fractions managed to increase NO production only at the highest concentration tested (100 μg/ml), while the neutral fraction IOE-WN activated potent NO production at 10 μg/ml and was considered the most promising immunomodulating fraction in this study.

KW - Chaga

KW - Fungi

KW - Polysaccharides

KW - Structural characterization

KW - Galactoglucan

KW - NMR

U2 - 10.1016/j.carbpol.2017.12.041

DO - 10.1016/j.carbpol.2017.12.041

M3 - Journal article

VL - 185

SP - 27

EP - 40

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -