Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy

S. Omarsdottir, Bent O. Petersen, H. Barsett, BS Paulsen, Jens Øllgaard Duus, ES Olafsdottir

Research output: Contribution to journalJournal articleResearchpeer-review


An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1 -> 6)-linked alpha-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of alpha-Manp and beta-Galp, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching. (c) 2005 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalCarbohydrate Polymers
Issue number1
Pages (from-to)54-60
Number of pages7
Publication statusPublished - 2006
Externally publishedYes


  • Ethanol
  • Molecular weight
  • Nuclear magnetic resonance
  • Spectroscopic analysis
  • Polysaccharides
  • Peltigera canina
  • Galactomannan
  • Lichens
  • Methylation analysis
  • NMR-spectroscopy
  • X
  • lichens
  • galactomannan
  • methylation analysis
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Peltigera canina species
  • Pc-4
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10511, Biophysics - Bioengineering
  • ethanol fractionation laboratory techniques
  • methanolysis laboratory techniques
  • methylation analysis laboratory techniques
  • NMR spectroscopy laboratory techniques, spectrum analysis techniques
  • size exclusion chromatography laboratory techniques, chromatographic techniques
  • Biomaterials


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