Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy

S. Omarsdottir; Bent O. Petersen; H. Barsett; BS Paulsen; Jens Øllgaard Duus; ES Olafsdottir

doi:10.1016/j.carbpol.2005.07.023

Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy

S. Omarsdottir, Bent O. Petersen, H. Barsett, BS Paulsen, Jens Øllgaard Duus, ES Olafsdottir

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1 -> 6)-linked alpha-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of alpha-Manp and beta-Galp, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching. (c) 2005 Elsevier Ltd. All rights reserved.

Original language	English
Journal	Carbohydrate Polymers
Volume	63
Issue number	1
Pages (from-to)	54-60
Number of pages	7
ISSN	0144-8617
DOIs	https://doi.org/10.1016/j.carbpol.2005.07.023
Publication status	Published - 2006
Externally published	Yes

Keywords

Ethanol
Molecular weight
Nuclear magnetic resonance
Spectroscopic analysis
Polysaccharides
Peltigera canina
Galactomannan
Lichens
Methylation analysis
NMR-spectroscopy
X
CHEMISTRY,
POLYMER
CELL-WALL
POLYSACCHARIDES
COMPONENTS
SEPARATION
CLADONIA
lichens
galactomannan
methylation analysis
Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Peltigera canina species
Pc-4
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10511, Biophysics - Bioengineering
ethanol fractionation laboratory techniques
methanolysis laboratory techniques
methylation analysis laboratory techniques
NMR spectroscopy laboratory techniques, spectrum analysis techniques
size exclusion chromatography laboratory techniques, chromatographic techniques
Biomaterials

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10.1016/j.carbpol.2005.07.023

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title = "Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy",

abstract = "An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1 -> 6)-linked alpha-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of alpha-Manp and beta-Galp, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching. (c) 2005 Elsevier Ltd. All rights reserved.",

keywords = "Ethanol, Molecular weight, Nuclear magnetic resonance, Spectroscopic analysis, Polysaccharides, Peltigera canina, Galactomannan, Lichens, Methylation analysis, NMR-spectroscopy, X, CHEMISTRY,, POLYMER, CELL-WALL, POLYSACCHARIDES, COMPONENTS, SEPARATION, CLADONIA, lichens, galactomannan, methylation analysis, Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Peltigera canina species, Pc-4, 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines, 10511, Biophysics - Bioengineering, ethanol fractionation laboratory techniques, methanolysis laboratory techniques, methylation analysis laboratory techniques, NMR spectroscopy laboratory techniques, spectrum analysis techniques, size exclusion chromatography laboratory techniques, chromatographic techniques, Biomaterials",

author = "S. Omarsdottir and Petersen, {Bent O.} and H. Barsett and BS Paulsen and Duus, {Jens {\O}llgaard} and ES Olafsdottir",

year = "2006",

doi = "10.1016/j.carbpol.2005.07.023",

language = "English",

volume = "63",

pages = "54--60",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Pergamon Press",

number = "1",

}

Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy. / Omarsdottir, S.; Petersen, Bent O.; Barsett, H.; Paulsen, BS; Duus, Jens Øllgaard; Olafsdottir, ES.

In: Carbohydrate Polymers, Vol. 63, No. 1, 2006, p. 54-60.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy

AU - Omarsdottir, S.

AU - Petersen, Bent O.

AU - Barsett, H.

AU - Paulsen, BS

AU - Duus, Jens Øllgaard

AU - Olafsdottir, ES

PY - 2006

Y1 - 2006

N2 - An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1 -> 6)-linked alpha-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of alpha-Manp and beta-Galp, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching. (c) 2005 Elsevier Ltd. All rights reserved.

AB - An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1 -> 6)-linked alpha-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of alpha-Manp and beta-Galp, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching. (c) 2005 Elsevier Ltd. All rights reserved.

KW - Ethanol

KW - Molecular weight

KW - Nuclear magnetic resonance

KW - Spectroscopic analysis

KW - Polysaccharides

KW - Peltigera canina

KW - Galactomannan

KW - Lichens

KW - Methylation analysis

KW - NMR-spectroscopy

KW - X

KW - CHEMISTRY,

KW - POLYMER

KW - CELL-WALL

KW - POLYSACCHARIDES

KW - COMPONENTS

KW - SEPARATION

KW - CLADONIA

KW - lichens

KW - galactomannan

KW - methylation analysis

KW - Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Peltigera canina species

KW - Pc-4

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 10511, Biophysics - Bioengineering

KW - ethanol fractionation laboratory techniques

KW - methanolysis laboratory techniques

KW - methylation analysis laboratory techniques

KW - NMR spectroscopy laboratory techniques, spectrum analysis techniques

KW - size exclusion chromatography laboratory techniques, chromatographic techniques

KW - Biomaterials

U2 - 10.1016/j.carbpol.2005.07.023

DO - 10.1016/j.carbpol.2005.07.023

M3 - Journal article

VL - 63

SP - 54

EP - 60

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 1

ER -

Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy

Abstract

Keywords

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