Abstract
An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1 -> 6)-linked alpha-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of alpha-Manp and beta-Galp, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching. (c) 2005 Elsevier Ltd. All rights reserved.
| Original language | English |
|---|---|
| Journal | Carbohydrate Polymers |
| Volume | 63 |
| Issue number | 1 |
| Pages (from-to) | 54-60 |
| Number of pages | 7 |
| ISSN | 0144-8617 |
| DOIs | |
| Publication status | Published - 2006 |
| Externally published | Yes |
Keywords
- Ethanol
- Molecular weight
- Nuclear magnetic resonance
- Spectroscopic analysis
- Polysaccharides
- Peltigera canina
- Galactomannan
- Lichens
- Methylation analysis
- NMR-spectroscopy
- X
- CHEMISTRY,
- POLYMER
- CELL-WALL
- POLYSACCHARIDES
- COMPONENTS
- SEPARATION
- CLADONIA
- lichens
- galactomannan
- methylation analysis
- Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Peltigera canina species
- Pc-4
- 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
- 10511, Biophysics - Bioengineering
- ethanol fractionation laboratory techniques
- methanolysis laboratory techniques
- methylation analysis laboratory techniques
- NMR spectroscopy laboratory techniques, spectrum analysis techniques
- size exclusion chromatography laboratory techniques, chromatographic techniques
- Biomaterials