Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare

Majid Haddad Momeni; Christina M. Payne; Henrik Hansson; Nils Egil Mikkelsen; Jesper Svedberg; Åke Engström; Mats Sandgren; Gregg T. Beckham; Jerry Ståhlberg

doi:10.1074/jbc.M112.440891

Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare

Majid Haddad Momeni
, Christina M. Payne
, Henrik Hansson
, Nils Egil Mikkelsen
, Jesper Svedberg
, Åke Engström
, Mats Sandgren
, Gregg T. Beckham
, Jerry Ståhlberg

Swedish University of Agricultural Sciences
University of Kentucky
Uppsala University
Colorado School of Mines

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Background: Family 7 cellulases exhibit significant hydrolytic potential in cellulose degradation. Results: We report the Heterobasidion irregulare GH7 structure and compare it with other GH7 cellobiohydrolases with simulation. Conclusion: H. irregulare Cel7A exhibits intermediate dynamical and structural properties between Phanerochaete chrysosporium Cel7D and Hypocrea jecorina Cel7A. Significance: These results highlight regions of family 7 cellobiohydrolases important for carbohydrate processivity and association-dissociation rates on cellulose.

Original language	English
Journal	Journal of Biological Chemistry
Volume	288
Issue number	8
Pages (from-to)	5861-5872
Number of pages	12
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.M112.440891
Publication status	Published - 2013
Externally published	Yes

Keywords

Biofuel
Cellulase
Crystallography
Glycoside hydrolases
Molecular dynamics
Heterobasidion annosum
Hypocrea jecorina
Phanerochaete chrysosporium
Trichoderma reesei
Cellobiohydrolase

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10.1074/jbc.M112.440891

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Momeni, M. H., Payne, C. M., Hansson, H., Mikkelsen, N. E., Svedberg, J., Engström, Å., Sandgren, M., Beckham, G. T., & Ståhlberg, J. (2013). Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare. Journal of Biological Chemistry, 288(8), 5861-5872. https://doi.org/10.1074/jbc.M112.440891

@article{49baaa94730a4ee1b74724d707d6c275,

title = "Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare",

abstract = "Background: Family 7 cellulases exhibit significant hydrolytic potential in cellulose degradation. Results: We report the Heterobasidion irregulare GH7 structure and compare it with other GH7 cellobiohydrolases with simulation. Conclusion: H. irregulare Cel7A exhibits intermediate dynamical and structural properties between Phanerochaete chrysosporium Cel7D and Hypocrea jecorina Cel7A. Significance: These results highlight regions of family 7 cellobiohydrolases important for carbohydrate processivity and association-dissociation rates on cellulose.",

keywords = "Biofuel, Cellulase, Crystallography, Glycoside hydrolases, Molecular dynamics, Heterobasidion annosum, Hypocrea jecorina, Phanerochaete chrysosporium, Trichoderma reesei, Cellobiohydrolase",

author = "Momeni, \{Majid Haddad\} and Payne, \{Christina M.\} and Henrik Hansson and Mikkelsen, \{Nils Egil\} and Jesper Svedberg and Åke Engstr{\"o}m and Mats Sandgren and Beckham, \{Gregg T.\} and Jerry St{\aa}hlberg",

year = "2013",

doi = "10.1074/jbc.M112.440891",

language = "English",

volume = "288",

pages = "5861--5872",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "8",

}

Momeni, MH, Payne, CM, Hansson, H, Mikkelsen, NE, Svedberg, J, Engström, Å, Sandgren, M, Beckham, GT & Ståhlberg, J 2013, 'Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare', Journal of Biological Chemistry, vol. 288, no. 8, pp. 5861-5872. https://doi.org/10.1074/jbc.M112.440891

Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare. / Momeni, Majid Haddad; Payne, Christina M.; Hansson, Henrik et al.
In: Journal of Biological Chemistry, Vol. 288, No. 8, 2013, p. 5861-5872.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare

AU - Momeni, Majid Haddad

AU - Payne, Christina M.

AU - Hansson, Henrik

AU - Mikkelsen, Nils Egil

AU - Svedberg, Jesper

AU - Engström, Åke

AU - Sandgren, Mats

AU - Beckham, Gregg T.

AU - Ståhlberg, Jerry

PY - 2013

Y1 - 2013

N2 - Background: Family 7 cellulases exhibit significant hydrolytic potential in cellulose degradation. Results: We report the Heterobasidion irregulare GH7 structure and compare it with other GH7 cellobiohydrolases with simulation. Conclusion: H. irregulare Cel7A exhibits intermediate dynamical and structural properties between Phanerochaete chrysosporium Cel7D and Hypocrea jecorina Cel7A. Significance: These results highlight regions of family 7 cellobiohydrolases important for carbohydrate processivity and association-dissociation rates on cellulose.

AB - Background: Family 7 cellulases exhibit significant hydrolytic potential in cellulose degradation. Results: We report the Heterobasidion irregulare GH7 structure and compare it with other GH7 cellobiohydrolases with simulation. Conclusion: H. irregulare Cel7A exhibits intermediate dynamical and structural properties between Phanerochaete chrysosporium Cel7D and Hypocrea jecorina Cel7A. Significance: These results highlight regions of family 7 cellobiohydrolases important for carbohydrate processivity and association-dissociation rates on cellulose.

KW - Biofuel

KW - Cellulase

KW - Crystallography

KW - Glycoside hydrolases

KW - Molecular dynamics

KW - Heterobasidion annosum

KW - Hypocrea jecorina

KW - Phanerochaete chrysosporium

KW - Trichoderma reesei

KW - Cellobiohydrolase

U2 - 10.1074/jbc.M112.440891

DO - 10.1074/jbc.M112.440891

M3 - Journal article

SN - 0021-9258

VL - 288

SP - 5861

EP - 5872

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

ER -

Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare

Abstract

Keywords

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