Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare

Majid Haddad Momeni, Christina M. Payne, Henrik Hansson, Nils Egil Mikkelsen, Jesper Svedberg, Åke Engström, Mats Sandgren, Gregg T. Beckham, Jerry Ståhlberg

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Background: Family 7 cellulases exhibit significant hydrolytic potential in cellulose degradation. Results: We report the Heterobasidion irregulare GH7 structure and compare it with other GH7 cellobiohydrolases with simulation. Conclusion: H. irregulare Cel7A exhibits intermediate dynamical and structural properties between Phanerochaete chrysosporium Cel7D and Hypocrea jecorina Cel7A. Significance: These results highlight regions of family 7 cellobiohydrolases important for carbohydrate processivity and association-dissociation rates on cellulose.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume288
Issue number8
Pages (from-to)5861-5872
Number of pages12
ISSN0021-9258
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Biofuel
  • Cellulase
  • Crystallography
  • Glycoside hydrolases
  • Molecular dynamics
  • Heterobasidion annosum
  • Hypocrea jecorina
  • Phanerochaete chrysosporium
  • Trichoderma reesei
  • Cellobiohydrolase

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