Structural and foaming properties of whey and soy protein isolates in mixed systems before and after heat treatment

Alane Cangani Alves, Lara Martha, Federico Casanova, Guilherme M Tavares*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


The partial replacement of proteins from animal sources by plant proteins in formulated food products has been proposed as useful to improve sustainability aspects of the products without dramatically changing their techno-functional properties. Although several research groups have published on the gelling properties of mixed systems containing whey and soy protein isolates (WPI and SPI), their foaming properties are much less described. In this context, the main objective of this paper was to evaluate the structural and foaming properties of samples containing different mass ratios of WPI:SPI (100:0, 75:25, 50:50, 25:75 and 0:100) before and after heat treatment. The samples were evaluated according to their solubility, foaming capacity (FC), foam microstructure and foam stability (FS). Before heat treatment, mixing SPI to WPI did not affect the solubility of whey proteins, but, after heat treatment, insoluble co-aggregates were formed. Similar FC was measured for all samples despite their WPI:SPI ratio and the applied heat treatment. The partial replacement of WPI by SPI changed the microstructure of the foams and had an antagonistic effect on the FS of the samples, due to the negative effect of insoluble soy protein aggregates and/or insoluble co-aggregates on the reinforcement of the air-water interfacial film.
Original languageEnglish
JournalFood Science and Technology International
Issue number6
Pages (from-to)545-553
Number of pages9
Publication statusPublished - 2022


  • Whey protein isolate
  • Soy protein isolate
  • Co-aggregation
  • Mixed systems
  • Foaming properties


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