Streptocollin, a type IV lanthipeptide produced by Streptomyces collinus Tü 365

Dumitrita Iftime, Martin Jasyk, Andreas Kulik, Johannes F. Imhoff, Evi Stegmann, Wolfgang Wohlleben, Roderich D. Süssmuth, Tilmann Weber

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Lanthipeptides are ribosomally synthesized and posttranslationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of the class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL successfully triggered peptide production. Furthermore the streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152, resulting in 3.5-fold and 5.5-fold increased yields, respectively.The structure and ring topology of streptocollin were determined by high resolution MS/MS analyses. Streptocollin contains four macrocyclic rings, including one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.
Original languageEnglish
JournalChembiochem
Volume16
Issue number18
Pages (from-to)2615-2623
Number of pages9
ISSN1439-4227
DOIs
Publication statusPublished - 2015

Keywords

  • Type IV lanthipeptide
  • Natural products
  • Streptomyces
  • Genome mining
  • Lantibiotics

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