Abstract
Sonieated extracts of rhizobia, especiaUy Rhizobium leguminosarum from pea
and vetch, were run in horizontal starch gel electrophoresis in the cold. The rhizobia were
grown on agar on a slime suppressing substrate of tryptone-yeast extract-CaCl2 with small
amounts of mannitol, sorbitol and arabinose and other sugars as enzyme inducers. After electrophoresis
the gels were separated into several slabs by a gel cutter. Each slab was stained
for a particular enzyme. Among numerous enzyme systems tested we found useful variation
in esterases (EC 3.1.1.1, EC 3.1.1.2), 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30),
mannitol dehydrogenase (EC 1.1.1.67), and arabinose dehydrogenase (EC 1.1.1.46). It was
possible to distinguish at least 7 different types of pea rhizobia among 16 strains isolated from
one batch of 5 kg soil.
Original language | English |
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Journal | Plant and Soil |
Volume | 87 |
Issue number | 2 |
Pages (from-to) | 251-256 |
ISSN | 0032-079X |
DOIs | |
Publication status | Published - 1985 |