Strain identification in Rhizobium by starch gel electrophoresis of isoenzymes

Kjeld Christensen Engvild, G. Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Sonieated extracts of rhizobia, especiaUy Rhizobium leguminosarum from pea and vetch, were run in horizontal starch gel electrophoresis in the cold. The rhizobia were grown on agar on a slime suppressing substrate of tryptone-yeast extract-CaCl2 with small amounts of mannitol, sorbitol and arabinose and other sugars as enzyme inducers. After electrophoresis the gels were separated into several slabs by a gel cutter. Each slab was stained for a particular enzyme. Among numerous enzyme systems tested we found useful variation in esterases (EC 3.1.1.1, EC 3.1.1.2), 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), mannitol dehydrogenase (EC 1.1.1.67), and arabinose dehydrogenase (EC 1.1.1.46). It was possible to distinguish at least 7 different types of pea rhizobia among 16 strains isolated from one batch of 5 kg soil.
    Original languageEnglish
    JournalPlant and Soil
    Volume87
    Pages (from-to)251-256
    ISSN0032-079X
    Publication statusPublished - 1985

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