Sonieated extracts of rhizobia, especiaUy Rhizobium leguminosarum from pea and vetch, were run in horizontal starch gel electrophoresis in the cold. The rhizobia were grown on agar on a slime suppressing substrate of tryptone-yeast extract-CaCl2 with small amounts of mannitol, sorbitol and arabinose and other sugars as enzyme inducers. After electrophoresis the gels were separated into several slabs by a gel cutter. Each slab was stained for a particular enzyme. Among numerous enzyme systems tested we found useful variation in esterases (EC 184.108.40.206, EC 220.127.116.11), 3-hydroxybutyrate dehydrogenase (EC 18.104.22.168), mannitol dehydrogenase (EC 22.214.171.124), and arabinose dehydrogenase (EC 126.96.36.199). It was possible to distinguish at least 7 different types of pea rhizobia among 16 strains isolated from one batch of 5 kg soil.
|Journal||Plant and Soil|
|Publication status||Published - 1985|