Statistical Analysis of Pseudomonas aeruginosa Biofilm Development: Impact of Mutations in Genes Involved in Twitching Motility, Cell-to-Cell Signaling, and Stationary-Phase Sigma Factor Expression

Arne Heydorn, Bjarne Kjær Ersbøll, Junichi Kato, Morten Hentzer, Matthew R. Parsek, Tim Tolker-Nielsen, Michael Christian Givskov, Søren Molin

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Four strains of Pseudomonas aeruginosa (wild type, DeltapilHIJK mutant, lasI mutant, and rpoS mutant) were genetically tagged with the green fluorescent protein, and the development of flow chamber-grown biofilms by each of them was investigated by confocal laser scanning microscopy. The structural developments of the biofilms were quantified by the computer program COMSTAT (A. Heydorn, A. T. Nielsen, M. Hentzer, C. Sternberg, M. Givskov, B. K. Ersboll, and S. Molin, Microbiology 146:2395-2407, 2000). Two structural key variables, average thickness and roughness, formed the basis for an analysis of variance model comprising the four P. aeruginosa strains, five time points (55, 98, 146, 242, and 314 h), and three independent rounds of biofllm experiments. The results showed that the wild type, the DeltapilHIJK mutant, and the rpoS mutant display conspicuously different types of temporal biofilm development, whereas the lasI mutant was indistinguishable from the wild type at all time points. The wild type and the lasI mutant formed uniform, densely packed biofilms. The rpoS mutant formed densely packed biofilms that were significantly thicker than those of the wild type, whereas the DeltapilHIJK mutant formed distinct microcolonies that were regularly spaced and almost uniform in size. The results are discussed in relation to the current model of P. aeruginosa biofilm development.
    Original languageEnglish
    JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
    Volume68
    Issue number4
    Pages (from-to)2008-2017
    ISSN0099-2240
    DOIs
    Publication statusPublished - 2002

    Cite this

    @article{49c8a7d9eccb4a1c92663c3ecd55e8cf,
    title = "Statistical Analysis of Pseudomonas aeruginosa Biofilm Development: Impact of Mutations in Genes Involved in Twitching Motility, Cell-to-Cell Signaling, and Stationary-Phase Sigma Factor Expression",
    abstract = "Four strains of Pseudomonas aeruginosa (wild type, DeltapilHIJK mutant, lasI mutant, and rpoS mutant) were genetically tagged with the green fluorescent protein, and the development of flow chamber-grown biofilms by each of them was investigated by confocal laser scanning microscopy. The structural developments of the biofilms were quantified by the computer program COMSTAT (A. Heydorn, A. T. Nielsen, M. Hentzer, C. Sternberg, M. Givskov, B. K. Ersboll, and S. Molin, Microbiology 146:2395-2407, 2000). Two structural key variables, average thickness and roughness, formed the basis for an analysis of variance model comprising the four P. aeruginosa strains, five time points (55, 98, 146, 242, and 314 h), and three independent rounds of biofllm experiments. The results showed that the wild type, the DeltapilHIJK mutant, and the rpoS mutant display conspicuously different types of temporal biofilm development, whereas the lasI mutant was indistinguishable from the wild type at all time points. The wild type and the lasI mutant formed uniform, densely packed biofilms. The rpoS mutant formed densely packed biofilms that were significantly thicker than those of the wild type, whereas the DeltapilHIJK mutant formed distinct microcolonies that were regularly spaced and almost uniform in size. The results are discussed in relation to the current model of P. aeruginosa biofilm development.",
    author = "Arne Heydorn and Ersb{\o}ll, {Bjarne Kj{\ae}r} and Junichi Kato and Morten Hentzer and Parsek, {Matthew R.} and Tim Tolker-Nielsen and Givskov, {Michael Christian} and S{\o}ren Molin",
    year = "2002",
    doi = "10.1128/AEM.68.4.2008-2017.2002",
    language = "English",
    volume = "68",
    pages = "2008--2017",
    journal = "Applied and Environmental Microbiology",
    issn = "0099-2240",
    publisher = "American Society for Microbiology",
    number = "4",

    }

    Statistical Analysis of Pseudomonas aeruginosa Biofilm Development: Impact of Mutations in Genes Involved in Twitching Motility, Cell-to-Cell Signaling, and Stationary-Phase Sigma Factor Expression. / Heydorn, Arne; Ersbøll, Bjarne Kjær; Kato, Junichi; Hentzer, Morten; Parsek, Matthew R.; Tolker-Nielsen, Tim; Givskov, Michael Christian; Molin, Søren.

    In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 68, No. 4, 2002, p. 2008-2017.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Statistical Analysis of Pseudomonas aeruginosa Biofilm Development: Impact of Mutations in Genes Involved in Twitching Motility, Cell-to-Cell Signaling, and Stationary-Phase Sigma Factor Expression

    AU - Heydorn, Arne

    AU - Ersbøll, Bjarne Kjær

    AU - Kato, Junichi

    AU - Hentzer, Morten

    AU - Parsek, Matthew R.

    AU - Tolker-Nielsen, Tim

    AU - Givskov, Michael Christian

    AU - Molin, Søren

    PY - 2002

    Y1 - 2002

    N2 - Four strains of Pseudomonas aeruginosa (wild type, DeltapilHIJK mutant, lasI mutant, and rpoS mutant) were genetically tagged with the green fluorescent protein, and the development of flow chamber-grown biofilms by each of them was investigated by confocal laser scanning microscopy. The structural developments of the biofilms were quantified by the computer program COMSTAT (A. Heydorn, A. T. Nielsen, M. Hentzer, C. Sternberg, M. Givskov, B. K. Ersboll, and S. Molin, Microbiology 146:2395-2407, 2000). Two structural key variables, average thickness and roughness, formed the basis for an analysis of variance model comprising the four P. aeruginosa strains, five time points (55, 98, 146, 242, and 314 h), and three independent rounds of biofllm experiments. The results showed that the wild type, the DeltapilHIJK mutant, and the rpoS mutant display conspicuously different types of temporal biofilm development, whereas the lasI mutant was indistinguishable from the wild type at all time points. The wild type and the lasI mutant formed uniform, densely packed biofilms. The rpoS mutant formed densely packed biofilms that were significantly thicker than those of the wild type, whereas the DeltapilHIJK mutant formed distinct microcolonies that were regularly spaced and almost uniform in size. The results are discussed in relation to the current model of P. aeruginosa biofilm development.

    AB - Four strains of Pseudomonas aeruginosa (wild type, DeltapilHIJK mutant, lasI mutant, and rpoS mutant) were genetically tagged with the green fluorescent protein, and the development of flow chamber-grown biofilms by each of them was investigated by confocal laser scanning microscopy. The structural developments of the biofilms were quantified by the computer program COMSTAT (A. Heydorn, A. T. Nielsen, M. Hentzer, C. Sternberg, M. Givskov, B. K. Ersboll, and S. Molin, Microbiology 146:2395-2407, 2000). Two structural key variables, average thickness and roughness, formed the basis for an analysis of variance model comprising the four P. aeruginosa strains, five time points (55, 98, 146, 242, and 314 h), and three independent rounds of biofllm experiments. The results showed that the wild type, the DeltapilHIJK mutant, and the rpoS mutant display conspicuously different types of temporal biofilm development, whereas the lasI mutant was indistinguishable from the wild type at all time points. The wild type and the lasI mutant formed uniform, densely packed biofilms. The rpoS mutant formed densely packed biofilms that were significantly thicker than those of the wild type, whereas the DeltapilHIJK mutant formed distinct microcolonies that were regularly spaced and almost uniform in size. The results are discussed in relation to the current model of P. aeruginosa biofilm development.

    U2 - 10.1128/AEM.68.4.2008-2017.2002

    DO - 10.1128/AEM.68.4.2008-2017.2002

    M3 - Journal article

    VL - 68

    SP - 2008

    EP - 2017

    JO - Applied and Environmental Microbiology

    JF - Applied and Environmental Microbiology

    SN - 0099-2240

    IS - 4

    ER -