Staphylococcus epidermidis recovered from indwelling catheters exhibit enhanced biofilm dispersal and "self-renewal" through downregulation of agr

Lu Dai, Liang Yang, Chris Parsons, Victoria J. Findlay, Søren Molin, Zhiqiang Qin

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Abstract

Background: In recent years, Staphylococcus epidermidis (Se) has become a major nosocomial pathogen and the most common cause of infections of implanted prostheses and other indwelling devices. This is due in part to avid biofilm formation by Se on device surfaces. However, it still remains unknown that how the process of Se biofilm development is associated with relapsed infection in such patients.Results: We have identified clinical Se isolates displaying enhanced biofilm dispersal and self-renewal relative to reference strain. These isolates also exhibit enhanced initial cell attachment, extracellular DNA release, cell autolysis and thicker microcolonies during biofilm development relative to reference strain. Our genetic analyses suggest that these clinical isolates exhibit significant downregulation of RNAIII, the effector molecule of the agr quorum sensing system, and upregulation of the autolysin gene atlE. Isogenic deletion of the agr system in Se 1457 confirmed that agr negatively regulating atlE resulted in enhanced initial cell attachment, extracellular DNA release, cell autolysis and biofilm formation abilities. In contrast, double deletion of agr and atlE significantly abolished these features.Conclusions: Collectively, these data reveal the role of agr system in long-term biofilm development and pathogenesis during Se caused indwelling devices-related relapsed infection.
Original languageEnglish
JournalB M C Microbiology
Volume12
Pages (from-to)Article No.: 102
ISSN1471-2180
DOIs
Publication statusPublished - 2012

Keywords

  • Gram-Positive Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Micrococcaceae [07702] Staphylococcus epidermidis species
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common
  • Staphylococcus epidermidis agr gene [Micrococcaceae]
  • Staphylococcus epidermidis atlE gene [Micrococcaceae]
  • Staphylococcus epidermidis RNAIII gene [Micrococcaceae]
  • extracellular DNA
  • polysaccharide intercellular adhesin PIA synthesis
  • 03502, Genetics - General
  • 03508, Genetics - Human
  • 12502, Pathology - General
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 36001, Medical and clinical microbiology - General and methods
  • 36002, Medical and clinical microbiology - Bacteriology
  • Human Medicine
  • Infection
  • Molecular Genetics
  • Staphylococcus epidermidis infection bacterial disease, infectious disease
  • biofilm dispersal
  • cell attachment
  • cell autolysis
  • self-renewalt
  • Biochemistry and Molecular Biophysics
  • Medical Sciences
  • genetic analysis laboratory techniques, genetic techniques

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