Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts

Shumin Zhang, Kai Xiong, Zhourui Xie, Wenting Nan, Honglin Liu, Jie Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-fetal fibroblast cells. Apart from high efficiency in the knockdown of BLG expression in these cells, lentivector-mediated RNAi manifested stable integration into the goat genome itself. Consequently, an in vitro model for goat BLG-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.

Original languageEnglish
JournalGenetics and Molecular Biology
Volume35
Issue number3
Pages (from-to)680-685
Number of pages6
ISSN1415-4757
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • BLG
  • Goat
  • Lentivirus
  • RNAi
  • shRNA

Fingerprint

Dive into the research topics of 'Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts'. Together they form a unique fingerprint.

Cite this