Stable nitrogen removal by anammox process after rapid temperature drops: Insights from metagenomics and metaproteomics

Sike Wang, Kento Ishii, Heng Yu, Xuchuan Shi, Barth F. Smets, Alejandro Palomo, Jiane Zuo*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study investigated the impacts of rapid temperature drops on anammox process performance and the metabolism of its core microbial populations through proteomic analysis. Over a 50-day period, the temperature of an up-flow granular bed anammox reactor was stepwise decreased from 35 °C to 15 °C and resulted in repeated transient increases in effluent nitrite concentrations. At 15 °C, a nitrogen removal rate of 2.71 ± 0.23 gN/(L·d) was maintained over 100 days operation. Total AnAOB population abundance (20.9%±4.9%) and AnAOB protein abundances (75.7% ± 3.3%) remained stable with decreased temperature. Key proteins of Ca. Brocadia for nitrogen metabolism, as well as for carbohydrate metabolism and primary metabolite biosynthesis were less expressed at 15 °C than 35 °C, while several proteins of heterotrophic Chloroflexi spp. involved in carbohydrate and metabolites metabolisms were expressed to a higher degree at 15 °C. Overall, metabolism of AnAOB responded at a higher degree to low temperatures than that of heterotrophs.

Original languageEnglish
Article number124231
JournalBioresource Technology
Volume320
ISSN0960-8524
DOIs
Publication statusPublished - 2021

Keywords

  • Anammox
  • Metabolisms
  • Metagenomics
  • Metaproteomics
  • Nitrogen removal

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