Stable isotope-resolved analysis with quantitative dissolution dynamic nuclear polarization

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Abstract

Metabolite profiles and their isotopomer distributions can be studied non-invasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such met-abolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ sig-nal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhance-ment, to facilitate reproducible and quantitative dDNP (qdDNP) NMR-based isotope tracer analysis. We further illustrate how qdDNP was applied to gain systematic and novel metabolic phenotypic insights into aggressive cancer cells.
Original languageEnglish
JournalAnalytical chemistry
Volume90
Issue number1
Pages (from-to)674–678
ISSN0003-2700
DOIs
Publication statusPublished - 2018

Cite this

@article{5be1958f222b4ef788683f9b04d1d887,
title = "Stable isotope-resolved analysis with quantitative dissolution dynamic nuclear polarization",
abstract = "Metabolite profiles and their isotopomer distributions can be studied non-invasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such met-abolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ sig-nal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhance-ment, to facilitate reproducible and quantitative dDNP (qdDNP) NMR-based isotope tracer analysis. We further illustrate how qdDNP was applied to gain systematic and novel metabolic phenotypic insights into aggressive cancer cells.",
author = "Lerche, {Mathilde Hauge} and Demet Yigit and Frahm, {Anne Birk} and Ardenkj{\ae}r-Larsen, {Jan Henrik} and Malinowski, {Ronja Maja} and Jensen, {Pernille Rose}",
year = "2018",
doi = "10.1021/acs.analchem.7b02779",
language = "English",
volume = "90",
pages = "674–678",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "1",

}

Stable isotope-resolved analysis with quantitative dissolution dynamic nuclear polarization. / Lerche, Mathilde Hauge; Yigit, Demet; Frahm, Anne Birk; Ardenkjær-Larsen, Jan Henrik; Malinowski, Ronja Maja; Jensen, Pernille Rose.

In: Analytical chemistry, Vol. 90, No. 1, 2018, p. 674–678.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Stable isotope-resolved analysis with quantitative dissolution dynamic nuclear polarization

AU - Lerche, Mathilde Hauge

AU - Yigit, Demet

AU - Frahm, Anne Birk

AU - Ardenkjær-Larsen, Jan Henrik

AU - Malinowski, Ronja Maja

AU - Jensen, Pernille Rose

PY - 2018

Y1 - 2018

N2 - Metabolite profiles and their isotopomer distributions can be studied non-invasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such met-abolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ sig-nal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhance-ment, to facilitate reproducible and quantitative dDNP (qdDNP) NMR-based isotope tracer analysis. We further illustrate how qdDNP was applied to gain systematic and novel metabolic phenotypic insights into aggressive cancer cells.

AB - Metabolite profiles and their isotopomer distributions can be studied non-invasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such met-abolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ sig-nal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhance-ment, to facilitate reproducible and quantitative dDNP (qdDNP) NMR-based isotope tracer analysis. We further illustrate how qdDNP was applied to gain systematic and novel metabolic phenotypic insights into aggressive cancer cells.

U2 - 10.1021/acs.analchem.7b02779

DO - 10.1021/acs.analchem.7b02779

M3 - Journal article

VL - 90

SP - 674

EP - 678

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 1

ER -