Stable isotope-resolved analysis with quantitative dissolution dynamic nuclear polarization

Mathilde Hauge Lerche, Demet Yigit, Anne Birk Frahm, Jan Henrik Ardenkjær-Larsen, Ronja Maja Malinowski, Pernille Rose Jensen

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Abstract

Metabolite profiles and their isotopomer distributions can be studied non-invasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such met-abolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ sig-nal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhance-ment, to facilitate reproducible and quantitative dDNP (qdDNP) NMR-based isotope tracer analysis. We further illustrate how qdDNP was applied to gain systematic and novel metabolic phenotypic insights into aggressive cancer cells.
Original languageEnglish
JournalAnalytical chemistry
Volume90
Issue number1
Pages (from-to)674–678
ISSN0003-2700
DOIs
Publication statusPublished - 2018

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