Stable and high-yielding intrinsic 59Fe-radiolabeling of the intravenous iron preparations Monofer and Cosmofer: Intrinsic 59Fe-radiolabeling of Monofer and Cosmofer

Andreas Tue Ingemann Jensen, Gregory Severin, Hans B. Andreasen, Palle Rasmussen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Commercial iron supplements Monofer((R)) and Cosmofer((R)) were intrinsically radiolabeled with Fe-59 for the purpose of tracing iron absorption in vivo. Optimized procedures aimed at introducing Fe-59 into the macromolecular construct in a form that was as chemically equivalent to the matrix iron as possible. This was determined by challenging the labeled constructs with diethylenetriaminepentaacetic acid (DTPA) followed by separation by size-exclusion and measurements of radioactivity and iron in the eluted fractions. The final procedures were simple and involved heating aqueous dispersions of the supplements in the presence of [Fe-59]FeCl3 for 24h at 95 degrees C for Monofer, and 85 degrees C for Cosmofer, resulting in radiochemical yields greater than 94%. High performance size exclusion chromatography, UV-VIS spectroscopy, and dynamic light scattering were used to show that the supplements remained unchanged after radiolabeling, underscoring the applicability of the methodology for radiolabeling commercial iron preparations.
    Original languageEnglish
    JournalJournal of Labelled Compounds and Radiopharmaceuticals
    Volume59
    Issue number9
    Pages (from-to)375-382
    Number of pages8
    ISSN0362-4803
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Intravenous iron therapy
    • Iron supplements
    • Monofer
    • Cosmofer
    • Fe-59
    • Intrinsic radiolabeling

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